Non-canonical pathway induced by Wnt3a regulates β-catenin via Pyk2 in differentiating human neural progenitor cells

被引：17
|
作者：
Talabattula, Venkata Ajay Narendra [1 ]
Morgan, Peter [1 ]
Frech, Moritz J. [1 ]
Uhrmacher, Adelinde M. [2 ]
Herchenroeder, Ottmar [3 ]
Puetzer, Brigitte M. [3 ]
Rolfs, Arndt [1 ]
Luo, Jiankai [1 ]
机构：
[1] Rostock Univ, Med Ctr, Albrecht Kossel Inst Neuroregenerat, Gehlsheimer Str 20, D-18147 Rostock, Germany
[2] Rostock Univ, Fac Comp Sci & Elect Engn, Med Ctr, Rostock, Germany
[3] Rostock Univ, Med Ctr, Inst Expt Gene Therapy & Canc Res, Rostock, Germany
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 491卷 / 01期
关键词：
Wnt3a; Calcium; Pyk2; beta-catenin; Differentiation; Human neural progenitor; GLYCOGEN-SYNTHASE KINASE-3; FOCAL TYROSINE KINASE; INTRACELLULAR CALCIUM; ACTIVATION; ADHESION; MECHANISM; FAMILY; FAK;
D O I：
10.1016/j.bbrc.2017.07.030
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Wnt/beta-catenin and Wnt/Ca2+ pathways are involved in cellular processes during embryonic development and the interaction between them in the same cell decides the outcome of cellular functions. In this study, we showed that Wnt3a triggers the Wnt/Ca2+ signaling pathway, indicated by an increase of cytosolic free calcium ([Ca2+](i)) and activation of calmodulin dependent kinase II (CaMKII) during the differentiation of human neuronal progenitor cells (hNPCs). Wnt3a via the increase of [Ca2+](i) activates proline-rich tyrosine kinase 2 (Pyk2), which subsequently regulates phosphorylation of glycogen synthase kinase 3 beta(GSK3 beta) and beta-catenin stabilization. Our findings suggest that Pyk2 plays an important role in the coordination of stabilization of P-catenin in the crosstalk between Wnt/beta-catenin and Wnt/ Ca2+ signaling pathways upon Wnt3a stimulation in differentiating hNPCs. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：40 / 46
页数：7
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