L-glutamine efficiently stimulates biosynthesis of bacillomycin D in Bacillus subtilis fmbJ

被引：12
|
作者：
Qian, Shiquan [1 ,2 ]
Sun, Jing [1 ]
Lu, Hedong [1 ]
Lu, Fengxia [1 ]
Bie, Xiaomei [1 ]
Lu, Zhaoxin [1 ]
机构：
[1] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China
[2] Bengbu Univ, Coll Food & Bioengn, Bengbu 233030, Peoples R China
来源：
PROCESS BIOCHEMISTRY | 2017年 / 58卷
基金：
中国国家自然科学基金;
关键词：
Amino acids; Bacillomycin D production; Fed-batch fermentation; Gene expression; SIGMA-FACTOR; ITURIN; ACID; ABRB; ANTIBIOTICS; LIPOPEPTIDE; INHIBITION; INITIATION; REGULATOR; CELLS;
D O I：
10.1016/j.procbio.2017.04.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study investigated the stimulation effect of L-glutamine (L-gln) on bacillomycin D biosynthesis of Bacillus subtilis fmbJ and the transcription of the synthetase genes and the signal proteins that related to the biosynthesis of bacillomycin D. It was found that L-gln significantly increased the production of bacillomycin D, in comparison with other amino acids. L-gln significantly enhanced the expression level of bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and up-regulated DegU, o(H) and SpoOA, but down-regulated AbrB, therefore the yield of bacillomycin D significantly increased. Furthermore, when conjointly feeding 5 g/L of L-gin and 30 g/L of inulin, the production of bacillomycin D was up to 1.93 g/L.
引用
收藏
页码：224 / 229
页数：6
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