This study was conducted to determine different phosphorus fractions in corn, cottonseed meal, dehulled soybean meal, fish meal analog, meat-bone meal, fish meal from menhaden Brevoortia spp., rice bran, sorghum, wheat, and wheat middlings, and phosphorus availability of these feedstuffs to channel catfish Ictalurus punctatus. Test diets consisting of a 70:30 mixture of reference diet to test ingredient were used with chromic oxide as the nondigestible marker. The various test diets were fed for 5 d in triplicate to groups of channel catfish weighing approximately 150-200 g, and the fecal samples were obtained by stripping approximately 12 h after feeding. Feed and fecal samples were analyzed for total phosphorus (TP) and soluble reactive phosphorus (SRP), from which apparent availability coefficients were calculated. Apparent availability of SRP exceeded 92% for all ingredients, except menhaden fish meal (61%) and wheat (44%). Total phosphorus availability from animal products was variable: 47% for menhaden fish meal, 54% for meat-bone meal, and 92% for fish meal analog. Plant products also had variable TP availability values: 33% for corn, 35% for both sorghum and soybean meal, 51% for rice bran, 66% for cottonseed meal, 77% for wheat middlings, and 83% for wheat. These phosphorus availability values obtained for the various feedstuffs may allow formulation of diets that are better utilized by channel catfish and reduce phosphorus excretion.