Ferrocene-mediated carbon paste electrode modified with D-fructose dehydrogenase for batch mode measurement of D-fructose

被引：10
|
作者：
Boujtita, M [1 ]
El Murr, N [1 ]
机构：
[1] Fac Sci Tech, CNRS UPRSA 6006, Grp Electrochim, Lab Anal Isotop & Electrochim Metab, F-44322 Nantes 03, France
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2000年 / 89卷 / 01期
关键词：
renewable surface electrode; modified carbon paste electrode; fructose dehydrogenase; fructose;
D O I：
10.1385/ABAB:89:1:55
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A mediated modified carbon paste and renewable surface electrode for fructose amperometric measurement based on D-fructose dehydrogenase (FDH) was prepared and optimized. Commercially available ferrocene (FcH) and hydroxymethyl ferrocene (FcCH(2)OH) were used as mediators. The substituted FcH showed better linearity and higher sensitivity. The influence of different experimental parameters was studied for optimum analytical performance. The final FDH-modified electrode showed good analytical performance for batch mode measurements of fructose.
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页码：55 / 66
页数：12
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