Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells Into Germ-Like Cells In Vitro

被引：143
|
作者：
Huang, Peng [1 ]
Lin, Li Min [1 ]
Wu, Xiao Ying [1 ]
Tang, Qiu Ling [1 ]
Feng, Xue Yong [1 ]
Lin, Guang Yu [1 ]
Lin, Xiaobo [1 ]
Wang, Hong Wu [1 ]
Huang, Tian Hua [2 ]
Ma, Lian [1 ]
机构：
[1] Shantou Univ, Coll Med, Affiliated Hosp 2, Dept Pediat, Shantou 515041, Guangdong, Peoples R China
[2] Shantou Univ, Coll Med, Res Ctr Reprod Med, Shantou 515041, Guangdong, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2010年 / 109卷 / 04期
基金：
中国国家自然科学基金;
关键词：
HUMAN UMBILICAL CORD; MESENCHYMAL STEM CELL; GERM LIKE CELLS; IN VITRO DIFFERENTIATION; ACID RECEPTOR-ALPHA; BONE-MARROW; RETINOIC ACID; DEVELOPMENTAL EXPRESSION; TRANSCRIPTION FACTOR; CONDITIONED MEDIUM; POTENTIAL ROLE; RAT TESTIS; MICE; DERIVATION;
D O I：
10.1002/jcb.22453
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed "tadpole-like" cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUE5), Ckit, CD49(f) (alpha 6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage. J. Cell. Biochem. 109: 747-754, 2010. (C) 2010 Wiley-Liss. Inc.
引用
收藏
页码：747 / 754
页数：8
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