Cloning and characterization of the ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10

被引：3

作者：

Liu, Xinyi ^{[1
]}

Wu, Haizhen ^{[1
]}

Ye, Jiang ^{[1
]}

Yuan, Oinsheng ^{[1
]}

Zhang, Huizhan ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2006年 / 52卷 / 12期

关键词：

ubiquinone; polyprenyl diphosphate synthase; gene expression; Rhodobacter capsulatus;

D O I：

10.1139/W06-080

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A decaprenyl diphosphate synthase gene (ddsA, GenBank accession No. DQ191802) was cloned from Rhodobacter capsulatus 1310 by constructing and screening the genome library. An open reading frame of 1002 bp was revealed from sequence analysis. The deduced polypeptide consisted of 333 amino acids residues with an molecular mass of about 37 kDa. The DdsA protein contained the conserved amino acid sequence (DDXXD) of E-type polyprenyl diphosphate synthase and showed high similarity to others. In contrast, DdsA showed only 39% identity to a solanesyl diphosphate synthase cloned from R. capsulatus SB1003. DdsA was expressed successfully in Escherichia coli. Assaying the enzyme in vivo found it made E.coli synthesize UQ-10 in addition to the endogenous production UQ-8.

引用

页码：1141 / 1147

页数：7

共 50 条

[41] Cloning and Characterization of Farnesyl Diphosphate Synthase Gene Involved in Triterpenoids Biosynthesis from Poria cocos
Wang, Jianrong
Li, Yangyuan
Liu, Danni
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (12) : 22188 - 22202
[42] A comparative study on the porins of Rhodobacter capsulatus strains 37b4 and B10 grown under different culture conditions
Robledano, M
Kleeberg, V
Kaiser, I
Benz, R
Schiltz, E
Weckesser, J
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (03) : 295 - 302
[43] Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata
Huang, Zhuoshi
Jiang, Keji
Pi, Yan
Hou, Rong
Liao, Zhihua
Cao, Ying
Han, Xu
Wang, Qian
Sun, Xiaofen
Tang, Kexuan
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 40 (05): : 625 - 635
[44] Biochemical, molecular, and genetic analyses of the acetone carboxylases from Xanthobacter autotrophicus strain Py2 and Rhodobacter capsulatus strain B10
Sluis, MK
Larsen, RA
Krum, JG
Anderson, R
Metcalf, WW
Ensign, SA
JOURNAL OF BACTERIOLOGY, 2002, 184 (11) : 2969 - 2977
[45] Gibberellin biosynthesis in Gibberella fujikuroi:: cloning and characterization of the copalyl diphosphate synthase gene
Tudzynski, B
Kawaide, H
Kamiya, Y
CURRENT GENETICS, 1998, 34 (03) : 234 - 240
[46] Gibberellin biosynthesis in Gibberella fujikuroi: cloning and characterization of the copalyl diphosphate synthase gene
B. Tudzynski
H. Kawaide
Y. Kamiya
Current Genetics, 1998, 34 : 234 - 240
[47] Cloning, overproduction and characterization of cytochrome c peroxidase from the purple phototrophic bacterium Rhodobacter capsulatus
De Smet, L
Pettigrew, GW
Van Beeumen, JJ
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (24): : 6559 - 6568
[48] Cloning, expression and characterization of a functional cDNA clone encoding geranylgeranyl diphosphate synthase of Hevea brasiliensis
Takaya, A
Zhanga, YW
Asawatreratanakul, K
Wititsuwannakul, D
Wititsuwannakul, R
Takahashi, S
Koyama, T
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2003, 1625 (02): : 214 - 220
[49] CLONING AND CHARACTERIZATION OF SENC, A GENE INVOLVED IN BOTH AEROBIC RESPIRATION AND PHOTOSYNTHESIS GENE-EXPRESSION IN RHODOBACTER-CAPSULATUS
BUGGY, J
BAUER, CE
JOURNAL OF BACTERIOLOGY, 1995, 177 (23) : 6958 - 6965
[50] Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus
Smith, CA
Suzuki, JY
Bauer, CE
PLANT MOLECULAR BIOLOGY, 1996, 30 (06) : 1307 - 1314

← 1 2 3 4 5 →