Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions

被引:6
|
作者
He, Jun [1 ]
Zhao, Yong [1 ]
Zhang, Shuangshuang [1 ]
He, Yanze [1 ]
Jiang, Jiafu [1 ]
Chen, Sumei [1 ]
Fang, Weimin [1 ]
Guan, Zhiyong [1 ]
Liao, Yuan [1 ]
Wang, Zhenxing [1 ]
Chen, Fadi [1 ]
Wang, Haibin [1 ]
机构
[1] Nanjing Agr Univ, Coll Hort,State Key Lab Crop Genet & Germplasm En, Natl Forestry & Grassland Adm,Key Lab Landscaping, Key Lab Biol Ornamental Plants East China,Minist, Nanjing 210095, Peoples R China
基金
中国国家自然科学基金;
关键词
Chrysanthemum; Asteraceae; 5S rDNA; 45S rDNA; oligonucleotide fluorescence in situ hybridization (Oligo-FISH); RIBOSOMAL-RNA GENES; REPETITIVE DNA; KARYOTYPE ANALYSIS; ASTERACEAE; EVOLUTION; SEQUENCE; FISH; REARRANGEMENTS; HYBRIDIZATION; LOCALIZATION;
D O I
10.3390/genes13050894
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
引用
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页数:14
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