Uneven Levels of 5S and 45S rDNA Site Number and Loci Variations across Wild Chrysanthemum Accessions

被引:6
|
作者
He, Jun [1 ]
Zhao, Yong [1 ]
Zhang, Shuangshuang [1 ]
He, Yanze [1 ]
Jiang, Jiafu [1 ]
Chen, Sumei [1 ]
Fang, Weimin [1 ]
Guan, Zhiyong [1 ]
Liao, Yuan [1 ]
Wang, Zhenxing [1 ]
Chen, Fadi [1 ]
Wang, Haibin [1 ]
机构
[1] Nanjing Agr Univ, Coll Hort,State Key Lab Crop Genet & Germplasm En, Natl Forestry & Grassland Adm,Key Lab Landscaping, Key Lab Biol Ornamental Plants East China,Minist, Nanjing 210095, Peoples R China
基金
中国国家自然科学基金;
关键词
Chrysanthemum; Asteraceae; 5S rDNA; 45S rDNA; oligonucleotide fluorescence in situ hybridization (Oligo-FISH); RIBOSOMAL-RNA GENES; REPETITIVE DNA; KARYOTYPE ANALYSIS; ASTERACEAE; EVOLUTION; SEQUENCE; FISH; REARRANGEMENTS; HYBRIDIZATION; LOCALIZATION;
D O I
10.3390/genes13050894
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Ribosomal DNA (rDNA) is an excellent cytogenetic marker owing to its tandem arrangement and high copy numbers. However, comparative studies have focused more on the number of rDNA site variations within the Chrysanthemum genus, and studies on the types of rDNA sites with the same experimental procedures at the species levels are lacking. To further explore the number and types of rDNA site variations, we combined related data to draw ideograms of the rDNA sites of Chrysanthemum accessions using oligonucleotide fluorescence in situ hybridization (Oligo-FISH). Latent variations (such as polymorphisms of 45S rDNA sites and co-localized 5S-45S rDNA) also occurred among the investigated accessions. Meanwhile, a significant correlation was observed between the number of 5S rDNA sites and chromosome number. Additionally, the clumped and concentrated geographical distribution of different ploidy Chrysanthemum accessions may significantly promote the karyotype evolution. Based on the results above, we identified the formation mechanism of rDNA variations. Furthermore, these findings may provide a reliable method to examine the sites and number of rDNA variations among Chrysanthemum and its related accessions and allow researchers to further understand the evolutionary and phylogenetic relationships of the Chrysanthemum genus.
引用
收藏
页数:14
相关论文
共 50 条
  • [1] High and uneven levels of 45S rDNA site-number variation across wild populations of a diploid plant genus (Anacyclus, Asteraceae)
    Rosato, Marcela
    Alvarez, Ines
    Nieto Feliner, Gonzalo
    Rossello, Josep A.
    [J]. PLOS ONE, 2017, 12 (10):
  • [2] Physical mapping of 5S and 45S rDNA loci in pufferfishes (Tetraodontiformes)
    Noleto, Rafael Bueno
    Vicari, Marcelo Ricardo
    Cipriano, Roger Raupp
    Artoni, Roberto Ferreira
    Cestari, Marta Margarete
    [J]. GENETICA, 2007, 130 (02) : 133 - 138
  • [3] Physical mapping of 5S and 45S rDNA loci in pufferfishes (Tetraodontiformes)
    Rafael Bueno Noleto
    Marcelo Ricardo Vicari
    Roger Raupp Cipriano
    Roberto Ferreira Artoni
    Marta Margarete Cestari
    [J]. Genetica, 2007, 130
  • [4] Physical mapping of the 5S and 45S rDNA in teosintes
    Han, YH
    Li, LJ
    Song, YC
    Li, ZY
    Xiong, ZY
    Li, DY
    [J]. HEREDITAS, 2002, 137 (01) : 16 - 19
  • [5] Physical mapping of 5S and 45S rDNA in Chrysanthemum and related genera of the Anthemideae by FISH, and species relationships
    MAGDY HUSSEIN ABD EL-TWAB
    KATSUHIKO KONDO
    [J]. Journal of Genetics, 2012, 91 : 245 - 249
  • [6] Physical mapping of 5S and 45S rDNA in Chrysanthemum and related genera of the Anthemideae by FISH, and species relationships
    Abd El-Twab, Magdy Hussein
    Kondo, Katsuhiko
    [J]. JOURNAL OF GENETICS, 2012, 91 (02) : 245 - 249
  • [7] Chromosomal Localization of the 5S and 45S rDNA Sites and 5S rDNA Sequence Analysis in Nelumbo Species
    Diao Ying
    Zhou Ming-quan
    Hu Zhong-li
    [J]. AGRICULTURAL SCIENCES IN CHINA, 2011, 10 (05): : 679 - 685
  • [8] Chromosomal Localization of the 5S and 45S rDNA Sites and 5S rDNA Sequence Analysis in Nelumbo Species
    DIAO YingZHOU Mingquan and HU Zhongli College of Life Science and TechnologyChongqing University of Arts and SciencesYongchuan PRChina Key Lab of Plant Developmental BiologyMinistry of EducationWuhan UniversityWuhan PRChina
    [J]. AgriculturalSciencesinChina, 2011, 10 (05) - 685
  • [9] Localization of 45S and 5S rDNA sites and karyotype of Chrysanthemum and its related genera by fluorescent in situ hybridization
    Qi, Xiangyu
    Zhang, Fei
    Guan, Zhiyong
    Wang, Haibin
    Jiang, Jiafu
    Chen, Sumei
    Chen, Fadi
    [J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2015, 62 : 164 - 172
  • [10] Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of Scadoxus multiflorus (Amaryllidaceae)
    Monkheang, Pansa
    Chaveerach, Arunrat
    Sudmoon, Runglawan
    Tanee, Tawatchai
    [J]. COMPARATIVE CYTOGENETICS, 2016, 10 (04) : 637 - 646