Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane

被引：10
|
作者：
Morton, April M.
Cunningham, Anthony L.
Diefenbach, Russell J. [1 ]
机构：
[1] Univ Sydney, Westmead Millennium Inst, Ctr Virus Res, Westmead, NSW 2145, Australia
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期
基金：
英国医学研究理事会;
关键词：
Kinesin-1; KIF5B; SNAP-25; Trafficking; Transport; HEAVY-CHAIN; CONVENTIONAL KINESIN; LIGHT-CHAIN; ANTEROGRADE TRANSPORT; AXONAL-TRANSPORT; TERMINAL DOMAIN; BINDING DOMAIN; CARGO-BINDING; PROTEIN; LOCALIZATION;
D O I：
10.1016/j.bbrc.2009.11.068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894 Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：388 / 393
页数：6
相关论文
共 50 条
  • [41] SNAP-25 with mutations in the zero layer supports normal membrane fusion kinetics
    Graham, ME
    Washbourne, P
    Wilson, MC
    Burgoyne, RD
    JOURNAL OF CELL SCIENCE, 2001, 114 (24) : 4397 - 4405
  • [42] Cysteine residues of SNAP-25 are required for SNARE disassembly and exocytosis, but not for membrane targeting
    Washbourne, P
    Cansino, V
    Mathews, JR
    Graham, M
    Burgoyne, RD
    Wilson, MC
    BIOCHEMICAL JOURNAL, 2001, 357 : 625 - 634
  • [43] A review of the role of synaptosomal-associated protein 25 (SNAP-25) in neurological disorders
    Noor, Aneeqa
    Zahid, Saadia
    INTERNATIONAL JOURNAL OF NEUROSCIENCE, 2017, 127 (09) : 805 - 811
  • [44] Cytotoxicity of botulinum neurotoxins reveals a direct role of syntaxin 1 and SNAP-25 in neuron survival
    Lisheng Peng
    Huisheng Liu
    Hongyu Ruan
    William H. Tepp
    William H. Stoothoff
    Robert H. Brown
    Eric A. Johnson
    Wei-Dong Yao
    Su-Chun Zhang
    Min Dong
    Nature Communications, 4
  • [45] Multimotor Transport in a System of Active and Inactive Kinesin-1 Motors
    Scharrel, Lara
    Ma, Rui
    Schneider, Rene
    Juelicher, Frank
    Diez, Stefan
    BIOPHYSICAL JOURNAL, 2014, 107 (02) : 365 - 372
  • [46] Cytotoxicity of botulinum neurotoxins reveals a direct role of syntaxin 1 and SNAP-25 in neuron survival
    Peng, Lisheng
    Liu, Huisheng
    Ruan, Hongyu
    Tepp, William H.
    Stoothoff, William H.
    Brown, Robert H.
    Johnson, Eric A.
    Yao, Wei-Dong
    Zhang, Su-Chun
    Dong, Min
    NATURE COMMUNICATIONS, 2013, 4
  • [47] Kinesin-1 and dynein transport Dense Core Vesicles in axons
    Lim, A.
    Saxton, W. M.
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22
  • [48] Kinesin-1 plays multiple roles during the vaccinia virus life cycle
    Schepis, Antonino
    Stauber, Tobias
    Krijnse Locker, Jacomine
    CELLULAR MICROBIOLOGY, 2007, 9 (08) : 1960 - 1973
  • [49] Role of SNAP-25 phosphorylation by protein kinase A in SNARE complex formation
    Gao, J.
    Takeuchi, H.
    Zhang, Z.
    Wang, D.
    FEBS JOURNAL, 2012, 279 : 381 - 381
  • [50] Axonal transport and targeting of the t-SNAREs SNAP-25 and syntaxin 1 in the peripheral nervous system
    Li, JY
    Jahn, R
    Dahlstrom, A
    EUROPEAN JOURNAL OF CELL BIOLOGY, 1996, 70 (01) : 12 - 22
12345