Evaluation of plastic scintillators for live imaging of 14C-labeled photosynthate movement in plants

被引:3
|
作者
Sugita, Ryohei [1 ]
Sugahara, Kohei [1 ]
Kobayashi, Natsuko I. [1 ]
Hirose, Atsushi [1 ]
Nakanishi, Tomoko M. [1 ]
Furuta, Etsuko [2 ]
Sensui, Masaaki [3 ]
Tanoi, Keitaro [1 ,4 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan
[2] Ochanomizu Univ, Fac Core Res, Bunkyo Ku, 2-1-1 Ohtsuka, Tokyo 1128610, Japan
[3] Tokyo Printing Ink Mfg Co Ltd, Kita Ku, 1-12-4 Ohji, Tokyo 1140002, Japan
[4] Sci & Technol Agcy JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan
基金
日本科学技术振兴机构;
关键词
Photosynthate; Live imaging; Real-time radioisotope imaging system; Plastic scintillator; SYSTEM; VISUALIZATION; ARABIDOPSIS; TRANSPORT; DYNAMICS; TISSUES; PHLOEM;
D O I
10.1007/s10967-018-6102-z
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We updated real-time radioisotope imaging system (RRIS) to study photosynthate movement in a large-size plant. Earlier, we used a CsI (Tl) scintillator attached to a fiber optic plate (FOS) to convert radiation into light. However, the scintillator size was fixed as 10 cm x 10 cm, which was too small to observe the entire plant. Moreover, the high cost of FOS makes it hard for other researchers to introduce the system in their facilities. Here, we investigated six types of low-priced plastic scintillators, and demonstrated the movement of C-14-labeled photosynthates in a living plant using the most efficient scintillator instead of FOS.
引用
收藏
页码:579 / 584
页数:6
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