Differential expression of multidrug resistance genes in naive rat brain

被引:33
|
作者
Kwan, P
Sills, GJ [1 ]
Butler, E
Gant, TW
Brodie, MJ
机构
[1] Univ Glasgow, Western Infirm, Dept Med & Therapeut, Epilepsy Unit, Glasgow G11 6NT, Lanark, Scotland
[2] Univ Leicester, MRC, Toxicol Unit, Leicester, Leics, England
关键词
multidrug resistance; P-glycoprotein; quantitative reverse-transcriptase polymerase chain reaction; brain; rat;
D O I
10.1016/S0304-3940(02)01453-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in naive rat brain. Adult male Sprague-Dawley rats (n = 6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:33 / 36
页数:4
相关论文
共 50 条
  • [21] Expression of genes implicated in multidrug resistance in acute lymphoblastic leukemia in India
    S. Gurbuxani
    D. Zhou
    G. Simonin
    V. Raina
    L. S. Arya
    S. Sazawal
    J.-P. Marie
    M. Bhargava
    Annals of Hematology, 1998, 76 : 195 - 200
  • [22] Expression of genes implicated in multidrug resistance in acute lymphoblastic leukemia in India
    Gurbuxani, S
    Zhou, D
    Simonin, G
    Raina, V
    Arya, LS
    Sazawal, S
    Marie, JP
    Bhargava, M
    ANNALS OF HEMATOLOGY, 1998, 76 (05) : 195 - 200
  • [23] EXPRESSION OF MULTIDRUG RESISTANCE GENES (MDR1) IN RHEUMATOID SYNOVIUM
    JORGENSEN, C
    ROSSI, JF
    BERNARD, JC
    CANOVAS, JF
    SIMONYLAFONTAINE, J
    RADAI, M
    REME, T
    SANY, J
    ARTHRITIS AND RHEUMATISM, 1992, 35 (09): : S201 - S201
  • [24] Differential expression of sirtuins in the aging rat brain
    Braidy, Nady
    Poljak, Anne
    Grant, Ross
    Jayasena, Tharusha
    Mansour, Hussein
    Chan-Ling, Tailoi
    Smythe, George
    Sachdev, Perminder
    Guillemin, Gilles J.
    FRONTIERS IN CELLULAR NEUROSCIENCE, 2015, 9
  • [25] Differential Expression of Axl and Correlation with Invasion and Multidrug Resistance in Cancer Cells
    Zhao, Yongfu
    Sun, Xiance
    Jiang, Lili
    Yang, Fengjuan
    Zhang, Zhaohai
    Jia, Li
    CANCER INVESTIGATION, 2012, 30 (04) : 287 - 294
  • [26] Multidrug resistance-associated protein and P-glycoprotein expression in rat brain microvessel endothelial cells
    Regina, A
    Koman, A
    Center, PS
    Couraud, PO
    Roux, F
    JOURNAL OF PHYSIOLOGY-LONDON, 1997, 505P : 57P - 57P
  • [27] Differential expression of three sialidase genes in rat development
    Hasegawa, T
    Carnero, CF
    Wada, T
    Itoyama, Y
    Miyagi, T
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 280 (03) : 726 - 732
  • [28] EXPRESSION OF MULTIDRUG-RESISTANCE GENES IN RAT-LIVER DURING REGENERATION AND AFTER CARBON-TETRACHLORIDE INTOXICATION
    NAKATSUKASA, H
    SILVERMAN, JA
    GANT, TW
    EVARTS, RP
    THORGEIRSSON, SS
    HEPATOLOGY, 1993, 18 (05) : 1202 - 1207
  • [29] EXPRESSION OF MULTIDRUG-RESISTANCE (MDR) AND GLUTATHIONE-TRANSFERASE-P (GST-P) GENES IN RAT HEPATOCARCINOGENESIS
    NAKATSUKASA, H
    EVARTS, RP
    SILVERMAN, JA
    MARSDEN, E
    THORGEIRSSON, SS
    FASEB JOURNAL, 1991, 5 (05): : A1247 - A1247
  • [30] EXPRESSION OF MULTIDRUG RESISTANCE AND GLUTATHIONE TRANSFERASE-PLACENTAL FORM GENES DURING CHEMICAL HEPATOCARCINOGENESIS IN RAT-LIVER
    NAKATSUKASA, H
    EVARTS, RP
    SILVERMAN, J
    THORGEIRSSON, SS
    HEPATOLOGY, 1991, 14 (04) : A108 - A108