Fibrinolytic, anti-inflammatory and anti-microbial properties of α-(1-3)-glucans produced from Streptococcus mutans (MTCC 497)

被引：30
|
作者：
Buddana, Sudheer Kumar [1 ,2 ]
Varanasi, Yaswanth Venkata Naga [1 ]
Shetty, Prakasham Reddy [1 ,2 ]
机构：
[1] Indian Inst Chem Technol, Hyderabad 500007, Andhra Pradesh, India
[2] Acad Sci & Innovat Res, New Delhi, India
来源：
CARBOHYDRATE POLYMERS | 2015年 / 115卷
关键词：
alpha-(1-3)-Glucans; Sulfated alpha-(1-3)-glucans; Antibacterial; Anti-inflammatory; Fibrinolytic; Streptococcus mutans; GLUCAN; PURIFICATION;
D O I：
10.1016/j.carbpol.2014.08.083
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Streptococcus mutans (MTCC 497) cell associated alpha-(1-3)-glucans were isolated, characterized and evaluated for their bioactivity profile. Acid hydrolysis of alpha-(1-3)-glucans revealed presence of glucose moieties. Water insoluble alpha-(1-3)-glucans (WIG) were sulfated to convert them into water soluble glucans which were characterized by FT-IR spectral studies. The sulfation of WIG was confirmed by the presence of -O-SO3- and C-O-SO3- characteristic peaks at 1240 and 820 cm(-1). MALDI-TOF analysis of sulfated alpha-(1-3)-glucan revealed 1.2 to 9 kDa fragmentation. Antibacterial profile studies revealed higher growth inhibitory activity against Gram negative than Gram positive bacterial strains by sulfated alpha-(1-3)-glucans. One-fold higher anti-inflammatory activity with IC50 value of 0.11 mg/ml was observed with sulfated alpha-(1-3)-glucans over WIG. Time dependent fibrinolytic potential without requirement of tissue plasminogen activators was observed for sulfated alpha-(1-3)-glucans. This is the first report demonstrating the fibrinolytic and anti-inflammatory property for sulfated alpha-(1-3)-glucans. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：152 / 159
页数：8
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