A method for measuring nitric oxide radical scavenging activity. Scavenging properties of sulfur-containing compounds

被引：27
|
作者：
Vriesman, MF [1 ]
Haenen, GRMM [1 ]
Westerveld, GJ [1 ]
Paquay, JBG [1 ]
Voss, HP [1 ]
Bast, A [1 ]
机构：
[1] Vrije Univ Amsterdam, Fac Chem, Dept Pharmacochem, Leiden Amsterdam Ctr Drug Res,Div Mol Pharmacol, NL-1081 HV Amsterdam, Netherlands
来源：
PHARMACY WORLD & SCIENCE | 1997年 / 19卷 / 06期
关键词：
nitric oxide radical; NO scavenging; thiol; S-nitrosothiol (electrochemical); NO sensing;
D O I：
10.1023/A:1008601327920
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A new method for the quantificatiion of the nitric oxide ((NO)-N-.) scavenging activity of compounds in aqueous solutions is describing using an amperometric (NO)-N-. sensor. After correction for the spontaneous degradation of (NO)-N-., second-order rate kinetics of the scavenging reactions are observe. The rate constant for hemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur-containing compounds were tested (GSH, GSSG, S-methyl glutathione, N-acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable (NO)-N-. scavenging activity.
引用
收藏
页码：283 / 286
页数：4
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