Pseudomonas aeruginosa Keratitis: Protease IV and PASP as Corneal Virulence Mediators

被引:28
|
作者
O'Callaghan, Richard [1 ]
Caballero, Armando [1 ]
Tang, Aihua [1 ]
Bierdeman, Michael [1 ]
机构
[1] Univ Mississippi, Med Ctr, Dept Microbiol & Immunol, Jackson, MS 39216 USA
关键词
Pseudomonas aeruginosa; bacterial keratitis; corneal virulence; protease IV (PIV); P. aeruginosa small protease (PASP); catalytic triad; corneal ulcer; respiratory infection; insect infection; plant infection; ALKALINE PROTEASE; CONTACT-LENS; APOLIPOPHORIN-III; DEFICIENT MUTANTS; SECRETED PROTEASE; LASA PROTEASE; EXOTOXIN-A; EXPRESSION; ELASTASE; GENE;
D O I
10.3390/microorganisms7090281
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas aeruginosa is a leading cause of bacterial keratitis, especially in users of contact lenses. These infections are characterized by extensive degradation of the corneal tissue mediated by Pseudomonas protease activities, including both Pseudomonas protease IV (PIV) and the P. aeruginosa small protease (PASP). The virulence role of PIV was determined by the reduced virulence of a PIV-deficient mutant relative to its parent strain and the mutant after genetic complementation (rescue). Additionally, the non-ocular pathogen Pseudomonas putida acquired corneal virulence when it produced active PIV from a plasmid-borne piv gene. The virulence of PIV is not limited to the mammalian cornea, as evidenced by its destruction of respiratory surfactant proteins and the cytokine interleukin-22 (IL-22), the key inducer of anti-bacterial peptides. Furthermore, PIV contributes to the P. aeruginosa infection of both insects and plants. A possible limitation of PIV is its inefficient digestion of collagens; however, PASP, in addition to cleaving multiple soluble proteins, is able to efficiently cleave collagens. A PASP-deficient mutant lacks the corneal virulence of its parent or rescue strain evidencing its contribution to corneal damage, especially epithelial erosion. Pseudomonas-secreted proteases contribute importantly to infections of the cornea, mammalian lung, insects, and plants.
引用
收藏
页数:17
相关论文
共 50 条
  • [21] Pseudomonas aeruginosa LasA protease and corneal infections
    Alionte, LG
    Cannon, BM
    White, CD
    Caballero, AR
    O'Callaghan, RJ
    Hobden, JA
    CURRENT EYE RESEARCH, 2001, 22 (04) : 266 - 271
  • [22] Effectiveness of protease inhibitors against protease IV of Pseudomonas aeruginosa
    Cannon, BM
    Moreau, JM
    Caballero, A
    Thibodeaux, BA
    Traidej, M
    Hill, JM
    Kuang, R
    He, S
    Groutas, WC
    O'Callaghan, RJ
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2000, 41 (04) : S154 - S154
  • [23] Pseudomonas aeruginosa Staphylolytic protease (elastase A) and ocular virulence
    Hobden, JA
    Alionte, LG
    Estrellas, PS
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1999, 40 (04) : S259 - S259
  • [24] The Lon Protease Is Essential for Full Virulence in Pseudomonas aeruginosa
    Breidenstein, Elena B. M.
    Janot, Laure
    Strehmel, Janine
    Fernandez, Lucia
    Taylor, Patrick K.
    Kukavica-Ibrulj, Irena
    Gellatly, Shaan L.
    Levesque, Roger C.
    Overhage, Joerg
    Hancock, Robert E. W.
    PLOS ONE, 2012, 7 (11):
  • [25] Pseudomonas aeruginosa LasA protease as a therapeutic agent in Staphylococcal keratitis
    Barequet, IS
    Safrin, M
    Bourla, N
    Ben Simon, GJ
    Ohman, DE
    Rosner, M
    Kessler, E
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 : U548 - U548
  • [26] Pseudomonas aeruginosa LasA protease in treatment of experimental staphylococcal keratitis
    Barequet, IS
    Ben Simon, GJ
    Safrin, M
    Ohman, DE
    Kessler, E
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (05) : 1681 - 1687
  • [27] Relationship of Virulence Factors and Clinical Features in Keratitis Caused by Pseudomonas aeruginosa
    Oka, Naoko
    Suzuki, Takashi
    Ishikawa, Eri
    Yamaguchi, Satoshi
    Hayashi, Naoki
    Gotoh, Naomasa
    Ohashi, Yuichi
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (11) : 6892 - 6898
  • [28] Molecular analysis of Pseudomonas aeruginosa protease IV expressed in Pseudomonas putida
    Traidej, M
    Caballero, AR
    Marquart, ME
    Thibodeaux, BA
    O'Callaghan, RJ
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 (01) : 190 - 196
  • [29] Condensins are essential for Pseudomonas aeruginosa corneal virulence through their control of lifestyle and virulence programs
    Zhao, Hang
    Clevenger, April L.
    Coburn, Phillip S.
    Callegan, Michelle C.
    Rybenkov, Valentin V.
    MOLECULAR MICROBIOLOGY, 2022, 117 (04) : 937 - 957
  • [30] Pseudomonas aeruginosa protease IV enzyme assays and comparison to other pseudomonas proteases
    Caballero, AR
    Moreau, JM
    Engel, LS
    Marquart, ME
    Hill, JM
    O'Callaghan, RJ
    ANALYTICAL BIOCHEMISTRY, 2001, 290 (02) : 330 - 337