Improved hematoxylin staining for algal cytogenetics

被引：8
|
作者：
Fujii, MT
Guerra, M [1 ]
机构：
[1] Univ Fed Pernambuco, Dept Bot, CCB, BR-50670420 Recife, PE, Brazil
[2] Inst Bot, Sect Phycol, BR-01061970 Sao Paulo, Brazil
来源：
BIOTECHNIC & HISTOCHEMISTRY | 1998年 / 73卷 / 02期
关键词：
algae; cytogenetics; hematoxylin; pit-connections;
D O I：
10.3109/10520299809140510
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aceto-iron hematoxylin method of Wittmann for algal chromosomes was improved by replacing the chloral hydrate and slide heating steps with 5 N HCl hydrolysis. Squashing of the unstained material under the coverslip followed by coverslip removal and air drying allowed rapid and more homogeneous staining. Additional procedures were developed to make the method easier and quicker. Good results were obtained for all algal species analyzed. Pit-connections of red algae were also clearly differentiated.
引用
收藏
页码：78 / 81
页数：4
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