Initial Response of Osteoblast-Like Cells on Magnesium Ion Implanted Titanium Surface

The interactions between the implant surface and surrounding tissue are important for successful osteoaenesis.1.3iomechanical interlocking and biochemical bonding are believed as to be the mechanism of osseomtegranon involving the initial osteoblast attachment, proliferation and differentiation. The purpose of this study is to evaluate the MC3T3-E1 cell responses to a magnesium (Mg) ion implanted titanium surface produced using a plasma source ion implantation method. Commercially pure titanium discs were prepared to produce tour different surfaces; ground surface, Ma-implanted on around surface, sandblasted surface, and Mg-implanted on sandblasted surface. Cell attachment, proliferation and differentiation of MC3T3-E1 cells were evaluated. Scanning electron microscopy and confocal laser microscopy revealed more cell proliferation on the Ma ion groups than on the non Ma ion groups On the ground surface, cells showed some degree of contact guidance in alignment with the grooves The MTT assay to determine the cellular viability and proliferation showed a higher optical density on the Mg ion aroups than on the non Mg ion groups This suggests that a Ma ion implanted titanium surface induces a better initial cellular response than the non Ma ion groups