A subcellular map of the human proteome

被引：1801

作者：

Thul, Peter J. ^{[1
]}

Akesson, Lovisa ^{[1
]}

Wiking, Mikaela ^{[1
]}

Mahdessian, Diana ^{[1
]}

Geladaki, Aikaterini ^{[2
,3
]}

Blal, Hammou Ait ^{[1
]}

Alm, Tove ^{[1
]}

Asplund, Anna ^{[4
]}

Bjork, Lars ^{[1
]}

Breckels, Lisa M. ^{[2
,5
]}

Backstrom, Anna ^{[1
]}

Danielsson, Frida ^{[1
]}

Fagerberg, Linn ^{[1
]}

Fall, Jenny ^{[1
]}

Gatto, Laurent ^{[2
,5
]}

Gnann, Christian ^{[1
]}

Hober, Sophia ^{[6
]}

Hjelmare, Martin ^{[1
]}

Johansson, Fredric ^{[1
]}

Lee, Sunjae ^{[1
]}

Lindskog, Cecilia ^{[4
]}

Mulder, Jan ^{[7
]}

Mulvey, Claire M. ^{[2
]}

Nilsson, Peter ^{[1
]}

Oksvold, Per ^{[1
]}

Rockberg, Johan ^{[6
]}

Schutten, Rutger ^{[1
]}

Schwenk, Jochen M. ^{[1
]}

Sivertsson, Asa ^{[1
]}

Sjostedt, Evelina ^{[4
]}

Skogs, Marie ^{[1
]}

Stadler, Charlotte ^{[1
]}

Sullivan, Devin P. ^{[1
]}

Tegel, Hanna ^{[6
]}

Winsnes, Casper ^{[1
]}

Zhang, Cheng ^{[1
]}

Zwahlen, Martin ^{[1
]}

Mardinoglu, Adil ^{[1
]}

Ponten, Fredrik ^{[4
]}

von Feilitzen, Kalle ^{[1
]}

Lilley, Kathryn S. ^{[2
]}

Uhlen, Mathias ^{[1
]}

Lundberg, Emma ^{[1
]}

机构：

[1] KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden

[2] Univ Cambridge, Dept Biochem, Cambridge Ctr Prote, Tennis Court Rd, Cambridge CB2 1QR, England

[3] Univ Cambridge, Dept Genet, Downing St, Cambridge CB2 3EH, England

[4] Uppsala Univ, Sci Life Lab, Genet & Pathol, Dept Immunol, SE-75185 Uppsala, Sweden

[5] Univ Cambridge, Dept Biochem, Computat Prote Unit, Tennis Court Rd, Cambridge CB2 1QR, England

[6] KTH Royal Inst Technol, Sch Biotechnol, Dept Prote, SE-10691 Stockholm, Sweden

[7] Karolinska Inst, Dept Neurosci, Sci Life Lab, SE-17177 Stockholm, Sweden

来源：

SCIENCE | 2017年 / 356卷 / 6340期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

SIGNAL PEPTIDES; HUMAN PROTEINS; LOCALIZATION; SINGLE; IMMUNOFLUORESCENCE; VALIDATION; PREDICTION; SELECTION; DYNAMICS; RESOURCE;

D O I：

10.1126/science.aal3321

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

引用

下载

页数：13

共 50 条

[21] Biologists initiate plan to map human proteome
Helen Pearson
Nature, 2008, 452 : 920 - 921
[22] Proteomics - Human proteome map not so distant
不详
CHEMISTRY & INDUSTRY, 2001, (08) : 231 - 231
[23] Tissue-based map of the human proteome
Uhlen, Mathias
Fagerberg, Linn
Hallstroem, Bjoern M.
Lindskog, Cecilia
Oksvold, Per
Mardinoglu, Adil
Sivertsson, Asa
Kampf, Caroline
Sjoestedt, Evelina
Asplund, Anna
Olsson, IngMarie
Edlund, Karolina
Lundberg, Emma
Navani, Sanjay
Szigyarto, Cristina Al-Khalili
Odeberg, Jacob
Djureinovic, Dijana
Takanen, Jenny Ottosson
Hober, Sophia
Alm, Tove
Edqvist, Per-Henrik
Berling, Holger
Tegel, Hanna
Mulder, Jan
Rockberg, Johan
Nilsson, Peter
Schwenk, Jochen M.
Hamsten, Marica
von Feilitzen, Kalle
Forsberg, Mattias
Persson, Lukas
Johansson, Fredric
Zwahlen, Martin
von Heijne, Gunnar
Nielsen, Jens
Ponten, Fredrik
SCIENCE, 2015, 347 (6220)
[24] A reference map of a human pituitary adenoma proteome
Zhan, XQ
Desiderio, DM
PROTEOMICS, 2003, 3 (05) : 699 - 713
[25] Biologists initiate plan to map human proteome
Pearson, Helen
NATURE, 2008, 452 (7190) : 920 - 921
[26] A cellular proteome map of human multiple myeloma
Drach, Johannes
Slany, Astrid
Sagaster, Verena
Gundacker, Nina
Haudek, Verena
Odelga, Viktona
Wimmer, Helge
Zielinski, Christoph
Gerner, Christopher
BLOOD, 2007, 110 (11) : 41A - 41A
[27] A CELLULAR PROTEOME MAP OF HUMAN MULTIPLE MYELOMA
Drach, W.
Slany, A.
Sagaster, V.
Gundacker, N.
Haudek, V.
Wimmer, H.
Zielinski, C.
Gerner, C.
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, 2008, 93 : 77 - 77
[28] Charting the subcellular proteome
Allison Doerr
Nature Methods, 2017, 14 : 650 - 650
[29] Charting the subcellular proteome
Doerr, Allison
NATURE METHODS, 2017, 14 (07) : 650 - 650
[30] Membrane protein; Detailed subcellular proteome study on human platelets
Thiele, H.
Wortelkamp, S.
Winckler, C.
Glandorf, J.
Sickmann, A.
MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (10) : S227 - S227

← 1 2 3 4 5 →