A subcellular map of the human proteome

被引：1801

作者：

Thul, Peter J. ^{[1
]}

Akesson, Lovisa ^{[1
]}

Wiking, Mikaela ^{[1
]}

Mahdessian, Diana ^{[1
]}

Geladaki, Aikaterini ^{[2
,3
]}

Blal, Hammou Ait ^{[1
]}

Alm, Tove ^{[1
]}

Asplund, Anna ^{[4
]}

Bjork, Lars ^{[1
]}

Breckels, Lisa M. ^{[2
,5
]}

Backstrom, Anna ^{[1
]}

Danielsson, Frida ^{[1
]}

Fagerberg, Linn ^{[1
]}

Fall, Jenny ^{[1
]}

Gatto, Laurent ^{[2
,5
]}

Gnann, Christian ^{[1
]}

Hober, Sophia ^{[6
]}

Hjelmare, Martin ^{[1
]}

Johansson, Fredric ^{[1
]}

Lee, Sunjae ^{[1
]}

Lindskog, Cecilia ^{[4
]}

Mulder, Jan ^{[7
]}

Mulvey, Claire M. ^{[2
]}

Nilsson, Peter ^{[1
]}

Oksvold, Per ^{[1
]}

Rockberg, Johan ^{[6
]}

Schutten, Rutger ^{[1
]}

Schwenk, Jochen M. ^{[1
]}

Sivertsson, Asa ^{[1
]}

Sjostedt, Evelina ^{[4
]}

Skogs, Marie ^{[1
]}

Stadler, Charlotte ^{[1
]}

Sullivan, Devin P. ^{[1
]}

Tegel, Hanna ^{[6
]}

Winsnes, Casper ^{[1
]}

Zhang, Cheng ^{[1
]}

Zwahlen, Martin ^{[1
]}

Mardinoglu, Adil ^{[1
]}

Ponten, Fredrik ^{[4
]}

von Feilitzen, Kalle ^{[1
]}

Lilley, Kathryn S. ^{[2
]}

Uhlen, Mathias ^{[1
]}

Lundberg, Emma ^{[1
]}

机构：

[1] KTH Royal Inst Technol, Sch Biotechnol, Sci Life Lab, SE-17121 Stockholm, Sweden

[2] Univ Cambridge, Dept Biochem, Cambridge Ctr Prote, Tennis Court Rd, Cambridge CB2 1QR, England

[3] Univ Cambridge, Dept Genet, Downing St, Cambridge CB2 3EH, England

[4] Uppsala Univ, Sci Life Lab, Genet & Pathol, Dept Immunol, SE-75185 Uppsala, Sweden

[5] Univ Cambridge, Dept Biochem, Computat Prote Unit, Tennis Court Rd, Cambridge CB2 1QR, England

[6] KTH Royal Inst Technol, Sch Biotechnol, Dept Prote, SE-10691 Stockholm, Sweden

[7] Karolinska Inst, Dept Neurosci, Sci Life Lab, SE-17177 Stockholm, Sweden

来源：

SCIENCE | 2017年 / 356卷 / 6340期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

SIGNAL PEPTIDES; HUMAN PROTEINS; LOCALIZATION; SINGLE; IMMUNOFLUORESCENCE; VALIDATION; PREDICTION; SELECTION; DYNAMICS; RESOURCE;

D O I：

10.1126/science.aal3321

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

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页数：13

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