miR-122 direct detection in human serum by time-gated fluorescence imaging

被引：15

作者：

Garcia-Fernandez, Emilio ^{[1
]}

Carmen Gonzalez-Garcia, M. ^{[1
]}

Pernagallo, Salvatore ^{[2
,3
]}

Ruedas-Rama, Maria J. ^{[1
]}

Fara, Mario A. ^{[2
]}

Lopez-Delgado, Francisco J. ^{[2
]}

Dear, James W. ^{[4
]}

Ilyine, Hugh ^{[3
]}

Ress, Cristina ^{[5
]}

Diaz-Mochon, Juan J. ^{[2
,6
,7
]}

Orte, Angel ^{[1
]}

机构：

[1] Univ Granada, Fac Farm, Dept Fisicoquim, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Campus Cartuja S-N, E-18071 Granada, Spain

[2] DestiNA Genom SL, Parque Tecnol Ciencias Salud PTS,Ave Innovac 1, Granada, Spain

[3] DestiNA Genom Ltd, 7-11 Melville St, Edinburgh EH3 7PE, Midlothian, Scotland

[4] Univ Edinburgh, Queens Med Res Inst, Ctr Cardiovasc Sci, 47 Little France Crescent, Edinburgh EH16 4TJ, Midlothian, Scotland

[5] Optoi Microelect, Via Vienna 8, I-38121 Trento, Gardolo, Italy

[6] Univ Granada, Andalusian Reg Govt, Ctr Genom & Oncol Res, GENYO, Ave Ilustrac 114, Granada 18016, Spain

[7] Univ Granada, Fac Farm, Dept Quim Farmaceut & Organ, Campus Cartuja S-N, E-18071 Granada, Spain

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 99期

基金：

欧盟地平线“2020”;

关键词：

CIRCULATING MICRORNAS; BIOMARKERS;

D O I：

10.1039/c9cc08069d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.

引用

页码：14958 / 14961

页数：4

共 50 条

[31] Inherent stochasticity, noise and limits of detection in continuous and time-gated fluorescence systems
Vitale, Nicholas H.
Hassibi, Arjang
Soh, Hyongsok Tom
Murmann, Boris
Lee, Thomas H.
PLOS ONE, 2024, 19 (12):
[32] Three-observation-window time-gated algorithm for fluorescence lifetime detection
Zhu, Sihui
Xu, Yue
Li, Ding
APPLIED OPTICS, 2020, 59 (09) : 2739 - 2745
[33] Multiplexed Time Lapse Fluorescence Lifetime Readouts in an Optically Sectioning Time-Gated Imaging Microscope
Margineanu, Anca
Laine, Romain
Kumar, Sunil
Talbot, Clifford
Warren, Sean
Kimberley, Christopher
McGinty, James
Kennedy, Gordon
Sardini, Alessandro
Dunsby, Christopher
Neil, Mark A. A.
Katan, Matilda
French, Paul M. W.
BIOPHYSICAL JOURNAL, 2011, 100 (03) : 183 - 183
[34] Detection of miR-122 by fluorescence real-time PCR in blood from patients with chronic hepatitis B and C infections
Ma, Zhen-Hua
Sun, Cheng-Xue
Shi, Hong
Fan, Jian-Hua
Song, Yu-Guo
Cong, Pei-Jun
Kong, Xiang-Min
Hao, Da-Lin
CYTOKINE, 2020, 131
[35] Ns-scaled time-gated fluorescence lifetime imaging for forensic document examination
Zhong, Xin
Wang, Xinwei
Zhou, Yan
2017 INTERNATIONAL CONFERENCE ON OPTICAL INSTRUMENTS AND TECHNOLOGY: OPTICAL SYSTEMS AND MODERN OPTOELECTRONIC INSTRUMENTS, 2017, 10616
[36] A Rapid Fluorescence Lifetime Image Acquistion Method Based on Time-gated Fluorescence Lifetime Imaging Microscopy
Liu, Yang
Zhou, Yan
Liu, Yuliang
2014 2ND INTERNATIONAL CONFERENCE ON SYSTEMS AND INFORMATICS (ICSAI), 2014, : 808 - 812
[37] True-color nanosecond time-gated fluorescence imaging using a CCD detector
Mitchell, A. C.
Wall, E. J.
Morgan, C. G.
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XIV, 2007, 6443
[38] Time-gated fluorescence signalling under dissipative conditions
Cardona, Maria A.
Chen, Rui
Maiti, Subhabrata
Fortunati, Ilaria
Ferrante, Camilla
Gabrielli, Luca
Das, Krishnendu
Prins, Leonard J.
CHEMICAL COMMUNICATIONS, 2020, 56 (90) : 13979 - 13982
[39] A Filterless Fluorescence Detector Based on a Time-Gated SiPM
Buonanno, Luca
Putelli, Chiara
Di Vita, Davide
Fiorini, Carlo
Carminati, Marco
2021 IEEE BIOMEDICAL CIRCUITS AND SYSTEMS CONFERENCE (IEEE BIOCAS 2021), 2021,
[40] Imaging of protein cluster sizes by means of confocal time-gated fluorescence anisotropy microscopy
Bader, Arjen N.
Hofman, Erik G.
Henegouwen, Paul M. P. van Bergen en
Gerritsen, Hans C.
OPTICS EXPRESS, 2007, 15 (11) : 6934 - 6945

← 1 2 3 4 5 →