miR-122 direct detection in human serum by time-gated fluorescence imaging

被引：15

作者：

Garcia-Fernandez, Emilio ^{[1
]}

Carmen Gonzalez-Garcia, M. ^{[1
]}

Pernagallo, Salvatore ^{[2
,3
]}

Ruedas-Rama, Maria J. ^{[1
]}

Fara, Mario A. ^{[2
]}

Lopez-Delgado, Francisco J. ^{[2
]}

Dear, James W. ^{[4
]}

Ilyine, Hugh ^{[3
]}

Ress, Cristina ^{[5
]}

Diaz-Mochon, Juan J. ^{[2
,6
,7
]}

Orte, Angel ^{[1
]}

机构：

[1] Univ Granada, Fac Farm, Dept Fisicoquim, Unidad Excelencia Quim Aplicada Biomed & Medioamb, Campus Cartuja S-N, E-18071 Granada, Spain

[2] DestiNA Genom SL, Parque Tecnol Ciencias Salud PTS,Ave Innovac 1, Granada, Spain

[3] DestiNA Genom Ltd, 7-11 Melville St, Edinburgh EH3 7PE, Midlothian, Scotland

[4] Univ Edinburgh, Queens Med Res Inst, Ctr Cardiovasc Sci, 47 Little France Crescent, Edinburgh EH16 4TJ, Midlothian, Scotland

[5] Optoi Microelect, Via Vienna 8, I-38121 Trento, Gardolo, Italy

[6] Univ Granada, Andalusian Reg Govt, Ctr Genom & Oncol Res, GENYO, Ave Ilustrac 114, Granada 18016, Spain

[7] Univ Granada, Fac Farm, Dept Quim Farmaceut & Organ, Campus Cartuja S-N, E-18071 Granada, Spain

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 99期

基金：

欧盟地平线“2020”;

关键词：

CIRCULATING MICRORNAS; BIOMARKERS;

D O I：

10.1039/c9cc08069d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.

引用

页码：14958 / 14961

页数：4

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