Head and neck lesions of Kimura's disease: Exclusion of human herpesvirus-8 and Epstein-Barr virus by in situ hybridisation and polymerase chain reaction. An immunohistochemical study

被引：15

作者：

Pitak-Arnnop, Poramate ^{[1
,2
,3
]}

Bellefqih, Salima ^{[4
,5
]}

Chaine, Andre ^{[1
]}

Dhanuthai, Kittipong ^{[6
]}

Bertrand, Jacques-Charles ^{[1
]}

Bertolus, Chloe ^{[1
]}

机构：

[1] Univ Paris 06, Pitie Salpetriere Univ Hosp, AP HP, Fac Med,Dept Maxillofacial Surg, Paris, France

[2] Univ Hosp Leipzig, Dept Oral Craniomaxillofacial & Facial Plast Surg, Fac Med, Leipzig, Germany

[3] Univ Paris 05, Lab Med Eth & Legal Med, Fac Med, Paris, France

[4] Maison Blanche Univ Hosp, Lab Pol Bouin, Dept Embryol Cytol Pathol & Mol Biol, Fac Med, Reims, France

[5] Univ Paris 06, Pitie Salpetriere Univ Hosp, AP HP, Dept Anat & Cytol Pathol,Fac Med, Paris, France

[6] Chulalongkorn Univ, Dept Oral Pathol, Fac Dent, Bangkok, Thailand

来源：

JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY | 2010年 / 38卷 / 04期

关键词：

Kimura's disease; head and neck lesions; immunohistochemistry; human herpesvisus-8; Epstein-Barr virus; KAPOSIS-SARCOMA; ANGIOLYMPHOID HYPERPLASIA; T-CELLS; SKIN; EXPRESSION; EOSINOPHILIA; DNA; CLONALITY; PATIENT; LATENT;

D O I：

10.1016/j.jcms.2009.08.001

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Introduction: Kimura's disease (KD) is a chronic inflammatory disorder, characterised by tumour-like lesions in the head and neck region, producing salivary gland nodules and lymph node enlargement. Many authors suggest that KD is a reactive immunological disorder; however, its aetiology remains unknown. Aims: To study immunohistochemical characteristics of head and neck lesions of KD (H&N-KD) and to investigate the possible role of human herpesvirus-8 (HHV-8) and Epstein-Barr virus (EBV) in the development of H&N-KD. Patients and methods: This study enrolled five H&N-KD specimens from three patients treated between 1995 and 2005 at Pitie-Salpetriere University Hospital, Paris, France. Immunohistochemical studies were performed on formalin-fixed, paraffin-embedded tissue. HHV-8 DNA was determined by polymerase chain reaction (PCR) analysis, whilst EBV sequences were identified by PCR and in situ hybridisation. Results: The immunohistochemical studies revealed CD20+ germinal centres with prominent staining of CD23+ dendritic reticular cells, surrounded by numerous interfollicular CD3+, and CD4+ or CD8+ T-cells. Factor VIII-related antigen, CD31 and CD34 occurred in the thin-walled blood vessels. The reactivity of CD1a, HHV-8 and EBV-associated latent membrane protein 1-EBV (LMP1-EBV) were negative, and in situ hybridisation confirmed the lack of EBV DNA. No patient recalled an external insult or chronic irritation. Conclusions: The results of this study indicate the reactive nature of H&N-KD (or a subset of H&N-KD), and it is unlikely that HHV-8 and EBV play a role in the pathogenesis of the lesion. However, the patients in this series did not have previous history of trauma or chronic irritation; thus, a neoplastic origin could not be excluded. Further multicentre studies based on more specimens are warranted. (C) 2009 European Association for Cranio-Maxillo-Facial Surgery

引用

页码：266 / 270

页数：5

共 31 条

[31] Epstein-Barr virus (EBV) is not significantly associated with oral squamous cell carcinomas (SCC) - A study using ligase dependent polymerase chain reaction (LD-PCR) and in-situ tissue hybridization to detect EBER-1.
Brandwein, M
Zhang, D
Urken, M
Berkowitz, D
Li, H
Punwaney, R
LABORATORY INVESTIGATION, 1997, 76 (01) : 656 - 656

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