Mechanism of ricin-induced apoptosis in human cervical cancer cells

被引:61
|
作者
Rao, PVL
Jayaraj, R
Bhaskar, ASB
Kumar, O
Bhattacharya, R
Saxena, P
Dash, PK
Vijayaraghavan, R
机构
[1] Def Res & Dev Estab, Div Pharmacol & Toxicol, Gwalior 474002, India
[2] Def Res & Dev Estab, Div Virol, Gwalior 474002, India
关键词
ricin; HeLa cells; apoptosis; caspase-3; DNA fragmentation factor; caspase inhibitors;
D O I
10.1016/j.bcp.2004.11.010
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The mechanism of ricin-induced apoptosis in human cervical cancer cell line HeLa was studied. The present study demonstrated that ricin induces apoptosis of human cervical cancer cells (HeLa) in a time dependent manner with an IC50 for cell viability of 1 mug/ml. Ricin treatment resulted in a time dependent increase in LDH leakage, DNA fragmentation, percent apoptotic cells, generation of reactive oxygen species and depletion of intracellular glutathione levels. DNA agarose gel electrophoresis showed typical oligonucleosomal length DNA fragmentation. Additionally, DNA diffusion assay was performed to confirm DNA damage and apoptosis. Ricin activated caspase-3 as evidenced by both proteolytic cleavage of procaspase-3 into 20 and 18 kDa subunits, and increased protease activity. Caspase activity was maximum at 4 h and led to the cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP), resulting in the 85 kDa cleavage product. Ricin-induced caspase-3 activation also resulted in cleavage of DNA fragmentation factor-45 (DFF45/ICAD) and DFF40 or caspase-activated DNase in HeLa cells. Activation of caspase-3, cleavage of PARP and DNA fragmentation was blocked by pre-treatment with caspase-3 specific inhibitor Ac-DEVD-CHO (100 muM) and broad-spectrum caspase inhibitor Z-VAD-FMK (40 muM). Ricin-induced DNA fragmentation was inhibited by pre-treatment with PARP inhibitors 3-aminobenzamide (100 muM) and DPQ (10 muM). Our results indicate that ricin-induced cell death was mediated by generation of reactive oxygen species and subsequent activation of caspase-3 cascade followed by down stream events leading to apoptotic mode of cell death. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:855 / 865
页数:11
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