Single-molecule mass spectrometry in solution using a solitary nanopore

被引：304
|
作者：
Robertson, Joseph W. F.
Rodrigues, Claudio G.
Stanford, Vincent M.
Rubinson, Kenneth A.
Krasilnikov, Oleg V. [1 ]
Kasianowicz, John J.
机构：
[1] Natl Inst Standards & Technol, Semicond Elect Div, Elect & Elect Engn Lab, Gaithersburg, MD 20899 USA
[2] Univ Fed Pernambuco, Dept Biophys & Radiobiol, Lab Membrane Biophys, BR-50670901 Recife, PE, Brazil
[3] Natl Inst Standards & Technol, Informat Access Div, Informat Technol Lab, Gaithersburg, MD 20899 USA
[4] Wright State Univ, Dept Biochem & Mol Biol, Dayton, OH 45435 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 20期
关键词：
Gaussian mixture model; poly(ethylene glycol); alpha-hemolysin; ion channel; maximum likelihood signal classification;
D O I：
10.1073/pnas.0611085104
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single a-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.
引用
收藏
页码：8207 / 8211
页数：5
相关论文
共 50 条
  • [31] Single-Molecule Picometer Resolution Nanopore Tweezers Resolution
    Gundlach, Jens H.
    Derrington, Ian M.
    Laszlo, Andrew H.
    Craig, Jonathan M.
    Nova, Ian C.
    Brinkerhoff, Henry
    Noakes, Mathew T.
    Ebright, Richard H.
    Mazumder, Abhishek
    BIOPHYSICAL JOURNAL, 2017, 112 (03) : 517A - 517A
  • [32] Nanopore confinement for electrochemical sensing at the single-molecule level
    Lin, Yao
    Ying, Yi-Lun
    Long, Yi-Tao
    CURRENT OPINION IN ELECTROCHEMISTRY, 2018, 7 : 172 - 178
  • [33] Reading Nanopore Clocks in Single-Molecule Electrophoresis Experiments
    Muthukumar, M.
    Katkar, H. H.
    BIOPHYSICAL JOURNAL, 2015, 108 (01) : 17 - 19
  • [34] Single-Molecule Study of Proteins by Biological Nanopore Sensors
    Wu, Dongmei
    Bi, Sheng
    Zhang, Liyu
    Yang, Jun
    SENSORS, 2014, 14 (10): : 18211 - 18222
  • [35] Nanopore single-molecule biosensor in protein denaturation analysis
    Sun, Hong
    Yao, Chuan
    You, Kaibo
    Chen, Can
    Liu, Shuoshuo
    Xu, Zhihong
    ANALYTICA CHIMICA ACTA, 2023, 1243
  • [36] Nanopore Cheminformatics: A new method for single-molecule biophysics
    Winters-Hilt, S
    DeGuzman, V
    Akeson, M
    BIOPHYSICAL JOURNAL, 2004, 86 (01) : 325A - 326A
  • [37] Single-Molecule Fingerprint Spectrum for Biological Nanopore Sensing
    Li, Xinyi
    Li, Meng-Yin
    Fu, Xixin
    Wan, Yongjing
    Ying, Yi-Lun
    Long, Yi-Tao
    BIOPHYSICAL JOURNAL, 2021, 120 (03) : 187A - 188A
  • [38] Learning Shapelets for Improving Single-Molecule Nanopore Sensing
    Wei, Zi-Xuan
    Ying, Yi-Lun
    Li, Meng-Yin
    Yang, Jie
    Zhou, Jia-Le
    Wang, Hui-Feng
    Yang, Bing-Yong
    Long, Yi-Tao
    ANALYTICAL CHEMISTRY, 2019, 91 (15) : 10033 - 10039
  • [39] Single-Molecule G-Quadruplex Nanopore Assay
    Boskovic, Filip N.
    Zhu, Jinbo
    Chen, Kaikai
    Keyser, Ulrich F.
    BIOPHYSICAL JOURNAL, 2020, 118 (03) : 466A - 466A
  • [40] Nanopore Single-Molecule Analysis of DNA Doxorubicin Interactions
    Yao, Fujun
    Duan, Jing
    Wang, Ying
    Zhang, Yue
    Guo, Yanli
    Guo, Huilin
    Kang, Xiaofeng
    ANALYTICAL CHEMISTRY, 2015, 87 (01) : 338 - 342
12345