Simple analysis of maleic hydrazide in agricultural products by HPLC

被引：5
|
作者：
Kobayashi, M [1 ]
Nagayama, T [1 ]
Takano, I [1 ]
Tamura, Y [1 ]
Tateishi, Y [1 ]
Tomizawa, S [1 ]
Kimura, N [1 ]
Kitayama, K [1 ]
Saito, K [1 ]
机构：
[1] Tokyo Metropolitan Res Lab Publ Hlth, Shinjuku Ku, Tokyo 1690073, Japan
来源：
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN | 2002年 / 43卷 / 06期
关键词：
maleic hydrazide; agricultural product; HPLC; herbicide; UV detector; LC/MS;
D O I：
10.3358/shokueishi.43.356
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A simplified HPLC determination method for maleic hydrazide in agricultural products was developed, and commercial agricultural crops were investigated. The homogenate of agricultural products was extracted with water. The crude extract was purified on an ACCUCAT Bond Elut(R) extraction cartridge using water. Maleic hydrazide was analyzed by HPLC with UV detection (303 nm). The HPLC separation was performed on a ZORBAX SB-Aq column with acetonitrile-water-phosphoric acid (5: 95: 0.01) as the mobile phase. Recoveries of maleic hydrazide from 15 agricultural products fortified at 1.0 and 10 mug/g were in the ranges of 92.6similar to104.9% and 94.2similar to101.3%, respectively. The limit of detection was 0.5 mug/g in samples. The proposed method was applied to the determination of 242 commercial vegetables and fruits. Maleic hydrazide was detected in 2 samples of imported onion at the levels of 4.9 and 7.2 mug/g.
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页码：356 / 361
页数：6
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