Edaravone improves the expression of nerve growth factor in human astrocytes subjected to hypoxia/reoxygenation

被引:9
|
作者
Yoshida, Hidemi [1 ]
Metoki, Norifumi [2 ]
Ishikawa, Akira [3 ]
Imaizumi, Tadaatsu [1 ]
Matsumiya, Tomoh [1 ]
Tanji, Kunikazu [4 ]
Ota, Ken [5 ]
Ohyama, Chikara [6 ]
Satoh, Kei [1 ]
机构
[1] Hirosaki Univ, Grad Sch Med, Dept Vasc Biol, Inst Brain Sci, Hirosaki, Aomori 0368562, Japan
[2] Hirosaki Stroke Ctr, Dept Internal Med, Hirosaki, Aomori 0368140, Japan
[3] Hirosaki Univ, Grad Sch Hlth Sci, Dept Disabil & Hlth, Hirosaki, Aomori 0368564, Japan
[4] Hirosaki Univ, Grad Sch Med, Dept Neuropathol, Inst Brain Sci, Hirosaki, Aomori 0368562, Japan
[5] Hirosaki Univ, Grad Sch Med, Dept Gastroenterol & Hematol, Hirosaki, Aomori 0368562, Japan
[6] Hirosaki Univ, Grad Sch Med, Dept Urol, Hirosaki, Aomori 0368562, Japan
关键词
Edaravone; NGF; Hypoxia; Ischemia; Reoxygenation; Reperfusion; FREE-RADICAL SCAVENGER; PROTEIN-KINASE PATHWAYS; CEREBRAL-ISCHEMIA; STRESS; PROLIFERATION; INFARCTION; HYPOXIA; JNK; SENESCENCE; MIGRATION;
D O I
10.1016/j.neures.2009.11.011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Edaravone (MCI-186, 3-methyl-1-phenyl-2-pyrazolin-5-one), a free radical scavenger, is known to ameliorate postischemic neuronal dysfunction. Nerve growth factor (NGF) is essential for neuronal growth and survival We have addressed the effect of edaravone on the NGF expression in astrocytes exposed to hypoxia/reoxygenation. Normal human astrocytes in culture were incubated under hypoxia for 3 h and then treated with edaravone tinder normal culture condition for up to 72 h The levels of NGF mRNA were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) or real-time quantitative PCR and NGF protein levels were measured by enzyme-linked immunosorbent assay (ELISA). Edaravone enhanced, in time- and concentration-dependent manners, the expressions of NGF mRNA and protein in astrocytes tinder reoxygenation condition. After the treatment for 72 h, 1 mmol/L edaravone enhanced the levels of NGF protein in astrocyte-conditioned media by 1.7-fold of the control An inhibitor of c-Jun N-terminal kinase (JNK) suppressed the effect of edaravone on the NGF expression, and cellular levels of phospho-JNK were increased in response to edaravone We conclude that edaravone enhances, via the JNK pathway, NGF expression in astrocytes This agent may exert a neurotrophic effect in the therapy of brain injury in ischemia/reperfusion. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
引用
收藏
页码:284 / 289
页数:6
相关论文
共 50 条
  • [31] The high expression of human β-nerve growth factor and biological activity demonstration
    Zhang, J
    Zhang, LY
    BRAIN RESEARCH, 1999, 848 (1-2) : A35 - A35
  • [32] EXPRESSION OF HUMAN NERVE GROWTH-FACTOR IN ESCHERICHIA-COLI
    SHIMIZU, N
    FUJIMORI, K
    FUKUZONO, S
    KOTOMURA, N
    FASEB JOURNAL, 1992, 6 (01): : A47 - A47
  • [33] EXPRESSION OF NERVE GROWTH-FACTOR RECEPTOR IN HUMAN PERIPHERAL NEUROPATHIES
    SOBUE, G
    YASUDA, T
    MITSUMA, T
    ROSS, AH
    PLEASURE, D
    ANNALS OF NEUROLOGY, 1988, 24 (01) : 64 - 72
  • [34] Regulation of nerve growth factor secretion and mRNA expression by bacterial lipopolysaccharide in primary cultures of rat astrocytes
    GalveRoperh, I
    Malpartida, JM
    Haro, A
    Brachet, P
    DiazLaviada, I
    JOURNAL OF NEUROSCIENCE RESEARCH, 1997, 49 (05) : 569 - 575
  • [35] DIFFERENTIAL EXPRESSION OF FIBROBLAST GROWTH-FACTOR RECEPTORS BY HUMAN NEURONS, ASTROCYTES AND MICROGLIA
    BALACI, L
    PRESTA, M
    ENNAS, MG
    DELLERA, P
    SOGOS, V
    LAURO, G
    GREMO, F
    NEUROREPORT, 1994, 6 (01) : 197 - 200
  • [36] Expression of nerve growth factor, nerve growth factor receptor and neuromodulin in regenerating muscle
    Heub, D
    Ganzmann, E
    Schellmann, U
    BRAIN PATHOLOGY, 1997, 7 (04) : 1119 - 1119
  • [37] Expression of nerve growth factor in hepatolithiasis
    Ohta, T
    Elnemr, A
    Yasui, T
    Kitagawa, H
    Kayahara, M
    Fushida, S
    Nishimura, G
    Nagakawa, T
    Miwa, K
    Yamamoto, M
    Terada, T
    Nakanuma, Y
    LIVER, 1999, 19 (06): : 489 - 494
  • [38] Hypoxia in human trophoblasts stimulates the expression and secretion of connective tissue growth factor
    Rimon, Eli
    Chen, Baosheng
    Shanks, Anthony L.
    Nelson, D. Michael
    Sadovsky, Yoel
    ENDOCRINOLOGY, 2008, 149 (06) : 2952 - 2958
  • [39] EXPRESSION OF NERVE GROWTH-FACTOR RECEPTOR DURING HUMAN PERIPHERAL-NERVE DEVELOPMENT
    SCARPINI, E
    ROSS, AH
    ROSEN, JL
    BROWN, MJ
    ROSTAMI, A
    KOPROWSKI, H
    LISAK, RP
    DEVELOPMENTAL BIOLOGY, 1988, 125 (02) : 301 - 310
  • [40] Altered plasma nerve growth factor-like immunoreactivity and nerve growth factor-receptor expression in human old age
    Antonelli, A
    Bracci-Laudiero, L
    Aloe, L
    GERONTOLOGY, 2003, 49 (03) : 185 - 190