Time-Resolved and Comprehensive Analysis of Surface Glycoproteins Reveals Distinct Responses of Monocytes and Macrophages to Bacterial Infection

被引：14
|
作者：
Suttapitugsakul, Suttipong [1 ,2 ]
Tong, Ming [1 ,2 ]
Wu, Ronghu [1 ,2 ]
机构：
[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
[2] Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 20期
基金：
美国国家卫生研究院;
关键词：
glycoproteins; immunology; membrane proteins; MS-based proteomics; time-resolved and comprehensive analysis; NF-KAPPA-B; CELL-SURFACE; INFLAMMATORY RESPONSES; PATHOGEN RECOGNITION; ADHESION MOLECULES; T-CELLS; LIPOPOLYSACCHARIDE; DIFFERENTIATION; EXPRESSION; BRAIN;
D O I：
10.1002/anie.202102692
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Glycoproteins on the surface of immune cells play extremely important roles in response to pathogens. Yet, a systematic and time-resolved investigation of surface glycoproteins during the immune response remains to be explored. Integrating selective enrichment of surface glycoproteins with multiplexed proteomics, we globally and site-specifically quantified the dynamics of surface glycoproteins on THP-1 monocytes and macrophages in response to bacterial infection and during the monocyte-to-macrophage differentiation. The time-resolved analysis reveals transient changes and differential remodeling of surface glycoproteins on both cell types, and potential upstream regulators and downstream effects of the regulated glycoproteins. Besides, we identified novel surface glycoproteins participating in the immune response such as APMAP, and site-specific changes of glycoproteins. This study provides unprecedented information to deepen our understanding of glycoproteins and cellular activities.
引用
收藏
页码：11494 / 11503
页数：10
相关论文
共 50 条
  • [41] Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat
    Jonathan J. Powell
    Jason Carere
    Gaurav Sablok
    Timothy L. Fitzgerald
    Jiri Stiller
    Michelle L. Colgrave
    Donald M. Gardiner
    John M. Manners
    John P. Vogel
    Robert J. Henry
    Kemal Kazan
    Scientific Reports, 7
  • [42] Time-Resolved Sum Frequency Generation Reveals Adsorbate Migration between Different Surface Active Sites on Titanium Oxide/Pt(111)
    Chen, Jun
    Kubota, Jun
    Wada, Akihide
    Kondo, Junko Nomura
    Domen, Kazunari
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (13) : 4580 - +
  • [43] Time-resolved and multi-tissue RNAseq provides new insights on the immune responses of European eels following infection with Aeromonas hydrophila
    Xiong, Fan
    Cao, Lu
    Xiong, Jing
    Wu, Ya Fang
    Huang, Wen Shu
    Chang, Ming Xian
    WATER BIOLOGY AND SECURITY, 2022, 1 (01):
  • [44] Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages
    Vogt, Carmen
    Pernemalm, Maria
    Kohonen, Pekka
    Laurent, Sophie
    Hultenby, Kjell
    Vahter, Marie
    Lehtio, Janne
    Toprak, Muhammet S.
    Fadeel, Bengt
    PLOS ONE, 2015, 10 (10):
  • [45] Transcriptome analysis of human macrophages upon chikungunya virus (CHIKV) infection reveals regulation of distinct signaling and metabolic pathways during the early and late stages of infection
    Srivastava, Priyanshu
    Chaudhary, Sakshi
    Malhotra, Surbhi
    Varma, Binuja
    Sunil, Sujatha
    HELIYON, 2023, 9 (06)
  • [46] Time-resolved single-cell analysis of Brca1 associated mammary tumourigenesis reveals aberrant differentiation of luminal progenitors
    Karsten Bach
    Sara Pensa
    Marija Zarocsinceva
    Katarzyna Kania
    Julie Stockis
    Silvain Pinaud
    Kyren A. Lazarus
    Mona Shehata
    Bruno M. Simões
    Alice R. Greenhalgh
    Sacha J. Howell
    Robert B. Clarke
    Carlos Caldas
    Timotheus Y. F. Halim
    John C. Marioni
    Walid T. Khaled
    Nature Communications, 12
  • [47] Time-resolved single-cell analysis of Brca1 associated mammary tumourigenesis reveals aberrant differentiation of luminal progenitors
    Bach, Karsten
    Pensa, Sara
    Zarocsinceva, Marija
    Kania, Katarzyna
    Stockis, Julie
    Pinaud, Silvain
    Lazarus, Kyren A.
    Shehata, Mona
    Simoes, Bruno M.
    Greenhalgh, Alice R.
    Howell, Sacha J.
    Clarke, Robert B.
    Caldas, Carlos
    Halim, Timotheus Y. F.
    Marioni, John C.
    Khaled, Walid T.
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [48] Integrated Analysis of Transcriptome and Metabolome Reveals Distinct Responses of Pelteobagrus fulvidraco against Aeromonas veronii Infection at Invaded and Recovering Stage
    Ning, Xianhui
    Peng, Ye
    Tang, Peng
    Zhang, Yiran
    Wang, Lingling
    Zhang, Wenwen
    Zhang, Kai
    Ji, Jie
    Yin, Shaowu
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (17)
  • [49] Kinetic Analysis of Local Oxygenation and Respiratory Responses of Mammalian Cells Using Intracellular Oxygen-Sensitive Probes and Time-Resolved Fluorometry
    Zhdanov, Alexander V.
    Dmitriev, Ruslan I.
    Hynes, James
    Papkovsky, Dmitri B.
    CONCEPTUAL BACKGROUND AND BIOENERGETIC/MITOCHONDRIAL ASPECTS OF ONCOMETABOLISM, 2014, 542 : 183 - 207
  • [50] Time-resolved quantitative analysis of human milk proteome reveals developing milk and mammary-gland functions during the first year of lactation
    Zhang, Qiang
    Cundiff, Judy K.
    Maria, Sarah D.
    McMahon, Robert J.
    Woo, Jessica G.
    Davidson, Barbara S.
    Morrow, Ardythe L.
    FASEB JOURNAL, 2013, 27
12345