Lunatibacter salilacus gen. nov., sp. nov., a member of the family Cyclobacteriaceae, isolated from a saline and alkaline lake sediment

A non-motile, Gram-staining negative, catalase- and oxidase-positive, crescent-rod shaped bacterium, designated strain CUG 91308(T), was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. Colonies on OSM agar were round, smooth, flat and pinkish-orange in colour. Strain CUG 91308(T) could grow at 15-37 degrees C, pH 6-12 and in the presence of up to 7.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91308(T) belonged to the family Cyclobacteriaceae and formed a clade with the genus Lunatimonas in the phylogenetic tree, but separated from any species of the known genera within the family. The genomic DNA G+C content is about 42.1 %. The predominant fatty acids (>10 %) were iso-C-15:0 (21.1%), summed feature 3 (C-16:1 omega 7c / C-16:1 omega 6c / iso-C-15:0 2OH) (14.3%), iso-C-17:0 3OH (12.3%) and summed feature 9 (iso-C-17:1 omega 9c / C-16:0 10-methyl) (10.6 %). The polar lipids of strain CUG 91308(T) were phosphatidylethanolamine (PE) and four unidentified polar lipids. Strain CUG 91308(T) contained MK-7 as the major respiratory quinone. On the basis of phenotypic, genotypic and phylogenetic data, strain CUG 91308(T) represents a novel species of a novel genus in the family Cyclobacteriaceae, for which the name Lunatibacter salilacus gen. nov., sp. nov. is proposed. The type strain of the proposed new isolate is CUG 91308(T) (=KCTC 62636(T)=CGMCC 1.13593(T)).