Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat

被引:108
|
作者
Oh, Seo Yeong [1 ]
Heo, Nam Su [1 ]
Shukla, Shruti [2 ]
Cho, Hye-Jin [3 ]
Vilian, A. T. Ezhil [2 ]
Kim, Jinwoon [1 ]
Lee, Sang Yup [4 ]
Han, Young-Kyu [2 ]
Yoo, Seung Min [4 ,5 ]
Huh, Yun Suk [1 ]
机构
[1] Inha Univ, Dept Chem Engn, 100 Inha Ro, Incheon 22212, South Korea
[2] Dongguk Univ Seoul, Dept Energy & Mat Engn, 30 Pildong Ro 1 Gil, Seoul 04620, South Korea
[3] Korea Res Inst Chem Technol KRICT, Reliabil Assessment Ctr Chem Mat, 141 Gajeong Ro, Daejeon 34114, South Korea
[4] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Plus Program BK21, Daejeon 34141, South Korea
[5] Chung Ang Univ, Sch Integrat Engn, 84 Heukseok Ro, Seoul 06974, South Korea
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
基金
新加坡国家研究基金会;
关键词
SURFACE-PLASMON RESONANCE; ELECTROCHEMICAL DETECTION; SENSITIVE DETECTION; BIOSENSOR; IMMUNOSENSOR; PLATFORM; SENSOR;
D O I
10.1038/s41598-017-10188-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A non-labeled, portable plasmonic biosensor-based device was developed to enable the ultra-sensitive and selective detection of Salmonella typhimurium in pork meat samples. Specifically, a plasmonic sensor, using the self-assembly of gold nanoparticles (AuNPs) to achieve a regulated diameter of 20 nm for the AuNP monolayers, was used to conduct high-density deposition on a transparent substrate, which produced longitudinal wavelength extinction shifts via a localized surface plasmon resonance (LSPR) signal. The developed aptamers conjugated to the LSPR sensing chips revealed an ultrasensitive upper limit of detection (LOD) of approximately 10(4) cfu/mL for S. typhimurium in pure culture under the optimal assay conditions, with a total analysis time of 30-35 min. When the LSPR sensing chips were applied on artificially contaminated pork meat samples, S. typhimurium in the spiked pork meat samples was also detected at an LOD of 1.0 x 10(4) cfu/mL. The developed method could detect S. typhimurium in spiked pork meat samples without a pre-enrichment step. Additionally, the LSPR sensing chips developed against S. typhimurium were not susceptible to any effect of the food matrix or background contaminant microflora. These findings confirmed that the developed gold nanoparticleaptamer- based LSPR sensing chips could facilitate sensitive detection of S. typhimurium in food samples.
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页数:10
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