Characterization of Acid- and Pepsin-soluble Collagens from Flatfish Skin

被引：50
|
作者：
Heu, Min Soo [2 ]
Lee, Jae Hyoung [1 ]
Kim, Hyung Jun [1 ]
Jee, Seong Joon [1 ]
Lee, Jung Suck [3 ]
Jeon, You-Jin [4 ]
Shahidi, Fereidoon [5 ]
Kim, Jin-Soo [1 ]
机构：
[1] Gyeongsang Natl Univ, Dept Seafood Sci & Technol, Inst Marine Ind, Tongyeong 650160, Gyeongnam, South Korea
[2] Gyeongsang Natl Univ, Dept Food Sci & Nutr, Inst Marine Ind, Jinju 660701, Gyeongnam, South Korea
[3] Aqua Green Technol Co Ltd, Cheju 690150, South Korea
[4] Jeju Natl Univ, Dept Marine Biotechnol, Cheju 690756, South Korea
[5] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9, Canada
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2010年 / 19卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
collagen; fish collagen; flatfish skin; flatfish; BIOCHEMICAL-PROPERTIES; I COLLAGEN; BLACK DRUM; FISH; GELATIN; MUSCLE; YOUNG; BONE;
D O I：
10.1007/s10068-010-0004-3
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from flatfish skin were characterized. The yield of PSC was 85.5%, which was higher than that of ASC at 57.3%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns showed that both the ASC and PSC were consisted of alpha 1 and alpha 2 chains, and beta-chain. According to the results of Fourier transform infrared (FTIR) spectroscopy and hydroxylation, no difference in the helical structure between ASC and PSC was found. Thermal denaturation temperature (TDT) of flatfish skin collagen was 26.6 degrees C for ASC and 26.7 degrees C for PSC, which were similar to those of temperate fishes, while were lower compared to tropical fishes, such as the conger eel and Nile perch. There was no solubility difference between ASC and PSC at various pH and NaCl concentrations. Therefore, PSC will be preferable compared to ASC for commercial uses because of its higher yield.
引用
收藏
页码：27 / 33
页数：7
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