Characterization of Acid- and Pepsin-soluble Collagens from Flatfish Skin

被引：50
|
作者：
Heu, Min Soo [2 ]
Lee, Jae Hyoung [1 ]
Kim, Hyung Jun [1 ]
Jee, Seong Joon [1 ]
Lee, Jung Suck [3 ]
Jeon, You-Jin [4 ]
Shahidi, Fereidoon [5 ]
Kim, Jin-Soo [1 ]
机构：
[1] Gyeongsang Natl Univ, Dept Seafood Sci & Technol, Inst Marine Ind, Tongyeong 650160, Gyeongnam, South Korea
[2] Gyeongsang Natl Univ, Dept Food Sci & Nutr, Inst Marine Ind, Jinju 660701, Gyeongnam, South Korea
[3] Aqua Green Technol Co Ltd, Cheju 690150, South Korea
[4] Jeju Natl Univ, Dept Marine Biotechnol, Cheju 690756, South Korea
[5] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9, Canada
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2010年 / 19卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
collagen; fish collagen; flatfish skin; flatfish; BIOCHEMICAL-PROPERTIES; I COLLAGEN; BLACK DRUM; FISH; GELATIN; MUSCLE; YOUNG; BONE;
D O I：
10.1007/s10068-010-0004-3
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from flatfish skin were characterized. The yield of PSC was 85.5%, which was higher than that of ASC at 57.3%. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns showed that both the ASC and PSC were consisted of alpha 1 and alpha 2 chains, and beta-chain. According to the results of Fourier transform infrared (FTIR) spectroscopy and hydroxylation, no difference in the helical structure between ASC and PSC was found. Thermal denaturation temperature (TDT) of flatfish skin collagen was 26.6 degrees C for ASC and 26.7 degrees C for PSC, which were similar to those of temperate fishes, while were lower compared to tropical fishes, such as the conger eel and Nile perch. There was no solubility difference between ASC and PSC at various pH and NaCl concentrations. Therefore, PSC will be preferable compared to ASC for commercial uses because of its higher yield.
引用
收藏
页码：27 / 33
页数：7
相关论文
共 50 条
  • [1] Characterization of acid- and pepsin-soluble collagens from flatfish skin
    Min Soo Heu
    Jae Hyoung Lee
    Hyung Jun Kim
    Seong Joon Jee
    Jung Suck Lee
    You-Jin Jeon
    Fereidoon Shahidi
    Jin-Soo Kim
    [J]. Food Science and Biotechnology, 2010, 19 : 27 - 33
  • [2] Isolation and characterization of acid- and pepsin-soluble collagens from Lagocephalus gloveri skin
    Kirti
    Samanta, Khora S.
    [J]. RESEARCH JOURNAL OF BIOTECHNOLOGY, 2015, 10 (12): : 22 - 28
  • [3] Characterization of Acid- and Pepsin-Soluble Collagens from the Cuticle of Perinereis nuntia (Savigny)
    Ao Liu
    Zhaohui Zhang
    Hu Hou
    Xue Zhao
    Bafang Li
    Tengfei Zhao
    Liangyu Liu
    [J]. Food Biophysics, 2018, 13 : 274 - 283
  • [4] Characterization of Acid- and Pepsin-Soluble Collagens from the Cuticle of Perinereis nuntia (Savigny)
    Liu, Ao
    Zhang, Zhaohui
    Hou, Hu
    Zhao, Xue
    Li, Bafang
    Zhao, Tengfei
    Liu, Liangyu
    [J]. FOOD BIOPHYSICS, 2018, 13 (03) : 274 - 283
  • [5] Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)
    Chuaychan, Sira
    Benjakul, Soottawat
    Kishimura, Hideki
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2015, 63 (01) : 71 - 76
  • [6] Physicochemical properties of acid- and pepsin-soluble collagens from the cartilage of Siberian sturgeon
    Luo, Qian-Bin
    Chi, Chang-Feng
    Yang, Fan
    Zhao, Yu-Qin
    Wang, Bin
    [J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2018, 25 (31) : 31427 - 31438
  • [7] Characterization of acid- and pepsin-soluble collagens from spines and skulls of skipjack tuna (Katsuwonus pelamis)
    Yu Di
    Chi Chang-Feng
    Wang Bin
    Ding Guo-Fang
    Li Zhong-Rui
    [J]. CHINESE JOURNAL OF NATURAL MEDICINES, 2014, 12 (09) : 712 - 720
  • [8] The Ecological Study of Isolation and Characterization of Acid and Pepsin-Soluble Collagens from the Skin of Lumpfish
    Zhuang, Xiaoli
    Bu, Honghong
    Zhou, Xiangzhu
    Dai, Xiaonan
    Li, Tianduo
    Wang, Liya
    [J]. EKOLOJI, 2018, 27 (106): : 2073 - 2081
  • [9] Physicochemical properties of acid- and pepsin-soluble collagens from the cartilage of Siberian sturgeon
    Qian-Bin Luo
    Chang-Feng Chi
    Fan Yang
    Yu-Qin Zhao
    Bin Wang
    [J]. Environmental Science and Pollution Research, 2018, 25 : 31427 - 31438
  • [10] Extraction and partial characterization of pepsin-soluble collagens from the skin of Amiurus nebulosus
    Chen, Lili
    Zhao, Li
    Liu, Hua
    Wu, Runfeng
    [J]. APPLICATION OF CHEMICAL ENGINEERING, PTS 1-3, 2011, 236-238 : 2926 - 2934
12345