A new host cell-line for the fast, reproducible and efficient production of biopharmaceuticals

被引：0
|
作者：
Greulich, B. [1 ]
Abts, H. F. [1 ]
Zahn, S. [1 ]
Herrmann, A. [1 ]
机构：
[1] Celon GmbH, Karl Heinz Beckurts Str 13, D-52428 Julich, Germany
来源：
CELL TECHNOLOGY FOR CELL PRODUCTS | 2007年
关键词：
recombinant protein expression; cell line generation; recombinase; biopharmaceuticals; production; FLP; resolvase;
D O I：
10.1007/978-1-4020-5476-1_140
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe here the proof of concept of a new, innovative "wildcard"-strategy (termed TaCEx-system, tag, characterize and exchange) for the generation of CHO-based cell-lines for the production of biopharmaceuticals using site-specific recombinase-mediated cassette exchange. Core element of the TaCEx-system is a pre-formed host cell-line. It contains a tagged reporter cassette by which the expression capacity of the integration site has been characterized and which allows the exchange of the reporter against a gene of interest (GOI). Such a new host cell facilitates the cloning of a GOI into predetermined genomic expression hot spots within days in order to get reproducible, highly efficient production cell-lines.
引用
收藏
页码：769 / +
页数：2
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