Expression of a MORN repeat protein from Euplotes octocarinatus requires a+1 programmed ribosomal frameshifting

被引：0
|
作者：
Wei, Lili [1 ]
Zhao, Xuemei [1 ]
Wang, Ruanlin [1 ]
Fu, Yuejun [1 ]
Chai, Baofeng [1 ]
Liang, Aihua [1 ]
机构：
[1] Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Taiyuan, Peoples R China
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2017年 / 81卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Euplotes octocarinatus; programmed ribosomal frameshifting; MORN; dual-luciferase experiment; mass spectrometry; GENE-EXPRESSION; MESSENGER-RNA; HIV-1; YEAST; TETRAHYMENA; EFFICIENCY; BACTERIAL; SLIPPAGE; SUPPORTS; HUMANS;
D O I：
10.1080/09168451.2017.1301804
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4-5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.
引用
收藏
页码：1327 / 1334
页数：8
相关论文
共 30 条
  • [21] STRUCTURE AND EXPRESSION OF THE GENE ENCODING RIBOSOMAL-PROTEIN-S1 FROM THE CYANOBACTERIUM SYNECHOCOCCUS SP STRAIN PCC-6301 - STRIKING SEQUENCE SIMILARITY TO THE CHLOROPLAST RIBOSOMAL PROTEIN-CS1
    SUGITA, M
    SUGITA, C
    SUGIURA, M
    MOLECULAR AND GENERAL GENETICS, 1995, 246 (02): : 142 - 147
  • [22] TRANSCRIPTION ACTIVATION OF YEAST RIBOSOMAL-PROTEIN GENES REQUIRES ADDITIONAL ELEMENTS APART FROM BINDING-SITES FOR ABF1P OR RAP1P
    GONCALVES, PM
    GRIFFIOEN, G
    MINNEE, R
    BOSMA, M
    KRAAKMAN, LS
    MAGER, WH
    PLANTA, RJ
    NUCLEIC ACIDS RESEARCH, 1995, 23 (09) : 1475 - 1480
  • [23] Expression of the translocator protein (TSPO) from Pseudomonas fluorescens Pf0-1 requires the stress regulatory sigma factors AlgU and RpoH
    Leneveu-Jenvrin, Charlene
    Bouffartigues, Emeline
    Maillot, Olivier
    Cornelis, Pierre
    Feuilloley, Marc G. J.
    Connil, Nathalie
    Chevalier, Sylvie
    FRONTIERS IN MICROBIOLOGY, 2015, 6
  • [24] TRANSACTIVATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LONG TERMINAL REPEAT-DIRECTED GENE-EXPRESSION BY THE HUMAN FOAMY VIRUS BEL1 PROTEIN REQUIRES A SPECIFIC DNA-SEQUENCE
    LEE, AH
    LEE, KJ
    KIM, SY
    SUNG, YC
    JOURNAL OF VIROLOGY, 1992, 66 (05) : 3236 - 3240
  • [25] ERYTHROID CELLS FROM DIAMOND BLACKFAN ANEMIA (DBA) PATIENTS WITH GATA1 AND RIBOSOMAL PROTEIN (RP) MUTATIONS SHOW MARKED DIFFERENCES IN RNA EXPRESSION
    Farrar, Jason
    O'Brien, Kelly
    Anderson, Stacie
    Atsidaftos, Eva
    Lipton, Jeffrey
    Ellis, Steven
    Bodine, David
    Vlachos, Adrianna
    PEDIATRIC BLOOD & CANCER, 2015, 62 : 28 - 28
  • [26] Impaired insulin secretion and decreased expression of the nutritionally responsive ribosomal kinase protein S6K-1 in pancreatic islets from malnourished rats
    Filiputti, Eliane
    Ferreira, Fabiano
    Souza, Kleber L. A.
    Stoppiglia, Luiz F.
    Arantes, Vanessa C.
    Boschero, Antonio C.
    Carneiro, Everardo M.
    LIFE SCIENCES, 2008, 82 (9-10) : 542 - 548
  • [27] DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae Encodes an RNA-Binding Protein from the Pentatricopeptide Repeat Family Required for the Maintenance of the Mitochondrial 15S Ribosomal RNA
    Puchta, Olga
    Lubas, Michal
    Lipinski, Kamil A.
    Piatkowski, Jakub
    Malecki, Michal
    Golik, Pawel
    GENETICS, 2010, 184 (04) : 959 - U112
  • [28] Ribosomal protein S26 (RPS26) expression of advanced cervical cancer (CC) to predict response to programmed death-1 inhibitor combination therapy: A secondary analysis of the CIBI308ALTER-C201 trial
    Liu, Jing
    Li, Li
    Chen, Ying
    Song, Yanwen
    Luo, Lan
    Zhu, Shitao
    Xu, Qin
    JOURNAL OF CLINICAL ONCOLOGY, 2023, 41 (16)
  • [29] INDUCTION OF BCL-2 EXPRESSION BY EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-1 PROTECTS INFECTED B-CELLS FROM PROGRAMMED CELL-DEATH
    HENDERSON, S
    ROWE, M
    GREGORY, C
    CROOMCARTER, D
    WANG, F
    LONGNECKER, R
    KIEFF, E
    RICKINSON, A
    CELL, 1991, 65 (07) : 1107 - 1115
  • [30] Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae
    Sundaresan, Ramya
    Samen, Ulrike
    Ponnuraj, Karthe
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 1582 - 1585
123