Expression of a MORN repeat protein from Euplotes octocarinatus requires a+1 programmed ribosomal frameshifting

被引：0

作者：

Wei, Lili ^{[1
]}

Zhao, Xuemei ^{[1
]}

Wang, Ruanlin ^{[1
]}

Fu, Yuejun ^{[1
]}

Chai, Baofeng ^{[1
]}

Liang, Aihua ^{[1
]}

机构：

[1] Shanxi Univ, Inst Biotechnol, Key Lab Chem Biol & Mol Engn, Minist Educ, Taiyuan, Peoples R China

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2017年 / 81卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Euplotes octocarinatus; programmed ribosomal frameshifting; MORN; dual-luciferase experiment; mass spectrometry; GENE-EXPRESSION; MESSENGER-RNA; HIV-1; YEAST; TETRAHYMENA; EFFICIENCY; BACTERIAL; SLIPPAGE; SUPPORTS; HUMANS;

D O I：

10.1080/09168451.2017.1301804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4-5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.

引用

页码：1327 / 1334

页数：8

共 30 条

[1] High frequency of +1 programmed ribosomal frameshifting in Euplotes octocarinatus
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[2] High frequency of+1 programmed ribosomal frameshifting in Euplotes octocarinatus
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[3] eIF5A promotes+1 programmed ribosomal frameshifting in Euplotes octocarinatus
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[4] Programmed translational frameshifting is likely required for expressions of genes encoding putative nuclear protein kinases of the ciliate Euplotes octocarinatus
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Liang, AH
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[8] Programmed-1 ribosomal frameshifting from the perspective of the conformational dynamics of mRNA and ribosomes
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[9] Expression and purification of a glutamic acid-rich protein from Euplotes octocarinatus in E. coli
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