In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

被引：0

作者：

Moura, Jusciene B. ^{[1
]}

de Vargas, Agueda C. ^{[2
]}

Gouveia, Gisele V. ^{[1
]}

Gouveia, Joao J. de S. ^{[1
]}

Ramos-Junior, Juracy C. ^{[3
]}

Botton, Sonia de A. ^{[2
]}

Pereira, Eugenia C. ^{[4
]}

da Costa, Mateus M. ^{[1
]}

机构：

[1] Univ Fed Vale Sao Francisco Univasf, Lab Microbiol & Imunol Anim, Campus Ciencias Rurais,BR 407 Km 12,Lote 543, BR-56300990 Petrolina, PE, Brazil

[2] Univ Fed Santa Maria, Dept Med Vet Prevent, Ctr Ciencias Rurais, Lab Bacteriol Labac, Av Roraima 1000,Predio 44,Sala 5125, BR-97105900 Santa Maria, RS, Brazil

[3] Clin Vet Sao Francisco Clivesf, Rua Aureliano Francisco Neto 38, BR-56308120 Petrolina, PE, Brazil

[4] Univ Fed Pernambuco UFPE, Dept Ciencias Geog, Ctr Filosofia & Ciencias Humanas, Av Acad Helio Ramos S-N,Cidade Univ, BR-50740907 Recife, PE, Brazil

来源：

PESQUISA VETERINARIA BRASILEIRA | 2017年 / 37卷 / 04期

关键词：

Antimicrobial activity; Cladonia substellata; lichens; usnic acid; Staphylococcus spp; cats; dogs; bacteria; biofilm; genes; ANTIBIOTIC-RESISTANCE; SECONDARY METABOLITES; BIOFILM FORMATION; OTITIS-EXTERNA; AUREUS; SUSCEPTIBILITY; EPIDERMIDIS; INTERMEDIUS; STRAINS; LICHENS;

D O I：

10.1590/S0100-736X2017000400011

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Cladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.

引用

页码：368 / 378

页数：11

共 31 条

[31] In-vitro activity of various antimicrobial peptides developed from the bactericidal domains of lysozyme and β-lactoglobulin with respect to Listeria monocytogenes, Escherichia coli O157, Salmonella spp. and Staphylococcus aureus
Pellegrini, A
Schumacher, S
Stephan, R
[J]. ARCHIV FUR LEBENSMITTELHYGIENE, 2003, 54 (02): : 34 - 36

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