Determination of cyanine-labeled amino acid enantiomers by cyclodextrin-modified capillary gel electrophoresis combined with diode laser fluorescence detection
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Kaneta, T
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Kyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, JapanKyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, Japan
Kaneta, T
[1
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Shiba, H
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Kyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, JapanKyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, Japan
Shiba, H
[1
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Imasaka, T
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Kyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, JapanKyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, Japan
Imasaka, T
[1
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[1] Kyushu Univ, Fac Engn, Dept Chem Sci & Technol, Higashi Ku, Fukuoka 812, Japan
Enantiomeric amino acids were labeled with a cyanine derivative (Cy5) for selective determination by capillary electrophoresis combined with diode laser-induced fluorometry. Cyanine-labeled glutamic acid has a high negative charge so that it was difficult to detect by conventional capillary zone electrophoresis due to its electrophoretic mobility being greater than the electroosmotic mobility. Thus, cyclodextrin-modified capillary gel electrophoresis using replaceable poly(vinylpyrrolidone) (PVP), which effectively suppresses the electroosmotic flow, was employed to separate Cy5-labeled amino acid enantiomers. The separation of six amino acid enantiomers could be achieved by using 1% PVP solution containing 70 mM gamma-cyclodextrin. It was also shown that PVP does not affect the selectivity of optical isomers. (C) 1998 Elsevier Science B.V.