Improved baculovirus system for transferring genes into insect and mammalian cells

被引：0
|
作者：
Koroleva, N. N. [1 ]
Grischuk, Yu. V. [1 ]
Kochetkova, S. V. [1 ]
Prasolov, V. S. [1 ]
Rubtsov, P. M. [1 ]
Beljelarskaya, S. N. [1 ]
机构：
[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 119991, Russia
来源：
MOLECULAR BIOLOGY | 2006年 / 40卷 / 06期
基金：
俄罗斯基础研究基金会;
关键词：
baculoviruses; insect cells; mammalian cells; gene expression; plasmid vectors; recombinant proteins;
D O I：
10.1134/S0026893306060173
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new set of eukaryotic expression vectors was constructed on the basis of baculoviruses. EcoRI fragments S, J, and P with the genes for late viral proteins p35 (polyhedrin), p39, and p10 were cloned from genomic DNA of the nuclear polyhedrosis virus. The promoter regions of these genes were used to construct double- and triple-promoter expression vectors. Baculovirus vectors containing an expression cassette with the cytomegalovirus promoter and the green fluorescent protein reporter gene were designed to express the cloned genes in cultured mammalian cells.
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页码：970 / 975
页数：6
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