Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Mercury Based on the Reaction between Alkaline Phosphatase and Mercury Using Triton X-100 as a Sensitizer

A new solid substrate room temperature phosphorimetry (SWIT) for the determination of trace mercury has been established, using Triton X-100 its it sensitizer The regression equation of working curve was Delta I-p = 11.40m(Hg2+)+1.569 (ao.spot(-1), n=6, Delta I-p=I-p1-I-p2, I-p1 and I-p2 referred to the phosphorescence intensity of the blank reagent and the test solution, respectively), and correlation coefficient (r) was 0.9984. The RSD valus of the determination of 0.016 and 8.0 ag.spot(-1) Hg2+ were 4.1% and 1.7% (n=8), respectively, indicating that the method had good repeatability. The limit of detection (LOD) calculated by 3Sb/k was 7.0 zg.spot(-1) Hg2+ (corresponding, concentration: 1.8 X 10(17) g.mL(-1), Sb = 0.025, n = 11). This method has high sensitivity, selectivity and precision, which wits applied to determination of trice mercury in water samples with the result being agreed very well with that of dithizone extraction spectrophotometry.