PCR-based diagnosis of infectious diseases

被引：0

作者：

Schewe, C ^{[1
]}

Rizzello, M ^{[1
]}

Dietel, M ^{[1
]}

Hauptmann, S ^{[1
]}

机构：

[1] Inst Pathol Charite, Rudolf Virchow Haus, D-10117 Berlin, Germany

来源：

PATHOLOGE | 2000年 / 21卷 / 03期

关键词：

infectious diseases; polymerase chain reaction; diagnostics; mycobacteria;

D O I：

暂无

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

PCR-based diagnosis of infectious diseases has developed into an important method in surgical pathology. We summarize our experience in this area based on 3500 analyses performed during the past 5 years. The peculiarity of material from a pathological laboratory is the risk of DNA fragmentation as a consequence of tissue fixation. For this reason the assays used must be adapted to this particular situation. PCR-based diagnosis of infectious diseases, if performed according to appropriate quality standards, is a safe and effective technique with high sensitivity and specificity and results in an etiologically based diagnosis.

引用

页码：218 / 228

页数：11

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