Colorimetric Sensing of α-Amino Acids and Its Application for the "Label-Free" Detection of Protease

被引：63
|
作者：
Lou, Xiaoding [2 ]
Zhang, Liyao [1 ]
Qin, Jingui [2 ]
Li, Zhen [2 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
[2] Wuhan Univ, Dept Chem, Hubei Key Lab Organ & Polymer Optoelect Mat, Wuhan 430072, Peoples R China
来源：
LANGMUIR | 2010年 / 26卷 / 03期
基金：
美国国家科学基金会;
关键词：
CONJUGATED POLYMERS; CHIRAL RECOGNITION; HIGH SELECTIVITY; FLUORESCENCE; SENSORS; WATER; HISTIDINE; PROBE; COORDINATION; CHEMOSENSOR;
D O I：
10.1021/la904138f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new indirect approach to explore sensitive colorimetric sensors toward alpha-amino acids is proposed: the pink solution of 1 and copper ions changed to colorless immediately upon the addition of alpha-amino acids. As the hydrolysis of bovine serum albumin (BSA) with the aid of trypsin produces alpha-amino acids, the complex of 1/Cu2+/BSA could act as a label-free, sensitive, selective sensor toward trypsin. The detection process could be visually observed by naked eyes.
引用
收藏
页码：1566 / 1569
页数：4
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