Quantitation of yeast ceramides using high-performance liquid chromatography-evaporative light-scattering detection

被引：18
|
作者：
Zhou, Q
Zhang, L
Fu, XQ
Chen, GQ [1 ]
机构：
[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 780卷 / 01期
关键词：
yeast cells; ceramides;
D O I：
10.1016/S1570-0232(02)00466-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC-ELSD) was used to assay the ceramides in yeast cells. The HPLC-ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：161 / 169
页数：9
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