Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards

被引:15
|
作者
Barth, Heidi [1 ]
Morel, Adrien [2 ]
Mougin, Christiane [2 ]
Averous, Gerlinde [3 ]
Legrain, Michele [4 ]
Fender, Muriel [5 ]
Risch, Simone [1 ]
Fafi-Kremer, Samira [1 ]
Velten, Michel [6 ]
Oudet, Pierre [7 ]
Baldauf, Jean-Jacques [2 ,8 ]
Stoll-Keller, Francoise [1 ]
机构
[1] Hop Univ Strasbourg, Virol Lab, Strasbourg, France
[2] Univ Franche Comte, CIC 1431, LabEx LipSTIC ANR 11 LABX 0021, EA 3181, F-25030 Besancon, France
[3] Hop Univ Strasbourg, Hop Hautepierre, Dept Pathol, Strasbourg, France
[4] Hop Univ Strasbourg, Hop Hautepierre, Lab Biochim & Biol Mol, Strasbourg, France
[5] EVE Assoc, Graffenstaden, France
[6] Univ Strasbourg, Fac Med, EA 3430, Lab Epidemiol & St Publ, Strasbourg, France
[7] Hop Univ Strasbourg, Hop Hautepierre, Canceropole Grand Est, Strasbourg, France
[8] Hop Univ Strasbourg, Hop Hautepierre, Dept Obstet Gynecol, Strasbourg, France
关键词
Papillomavirus; Cervical cancer; Storage of cells; Biobanking; CERVICAL-CANCER; FILTER-PAPER; GENOTYPE DISTRIBUTION; CYTOLOGIC SPECIMENS; CLINICAL-EVALUATION; MOLECULAR ANALYSIS; MULTIPLE TYPES; BLOOD SAMPLES; HPV; RISK;
D O I
10.1016/j.jviromet.2015.12.010
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that ETA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:60 / 65
页数:6
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