Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

被引：17

作者：

Todo, Kagefumi

Miyake, Kenji

Magari, Masaki

Kanayama, Naoki

Ohmori, Hitoshi ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Biotechnol, Okayama 7008530, Japan

[2] Japan Soc Promot Sci, Tokyo 1028472, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2006年 / 102卷 / 05期

基金：

日本学术振兴会;

关键词：

monoclonal antibody; chicken B cell line; hypermutation;

D O I：

10.1263/jbb.102.478

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

引用

页码：478 / 481

页数：4

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