Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

被引：17

作者：

Todo, Kagefumi

Miyake, Kenji

Magari, Masaki

Kanayama, Naoki

Ohmori, Hitoshi ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Biotechnol, Okayama 7008530, Japan

[2] Japan Soc Promot Sci, Tokyo 1028472, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2006年 / 102卷 / 05期

基金：

日本学术振兴会;

关键词：

monoclonal antibody; chicken B cell line; hypermutation;

D O I：

10.1263/jbb.102.478

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

引用

页码：478 / 481

页数：4

共 50 条

[1] Creation of Valuable Antibodies by an In Vitro Antibody Generation System Using a Hypermutating B Cell Line
Kanayama, Naoki
Todo, Kagefumi
Magari, Masaki
Ohmori, Hitoshi
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2009, 129 (01): : 11 - 17
[2] Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines
Sarah J. Cumbers
Gareth T. Williams
Sarah L. Davies
Richard L. Grenfell
Shunichi Takeda
Facundo D. Batista
Julian E. Sale
Michael S. Neuberger
Nature Biotechnology, 2002, 20 : 1129 - 1134
[3] Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines
Cumbers, SJ
Williams, GT
Davies, SL
Grenfell, RL
Takeda, S
Batista, FD
Sale, JE
Neuberger, MS
NATURE BIOTECHNOLOGY, 2002, 20 (11) : 1129 - 1134
[4] Two chicken B cell lines resistant to ouabain for the production of chicken monoclonal antibodies
Nishinaka, S
Akiba, H
Nakamura, M
Suzuki, K
Suzuki, T
Tsubokura, K
Horiuchi, H
Furusawa, S
Matsuda, H
JOURNAL OF VETERINARY MEDICAL SCIENCE, 1996, 58 (11): : 1053 - 1056
[5] A LIBRARY OF MONOCLONAL-ANTIBODIES AGAINST CHICKEN NUCLEAR ANTIGENS
LEHNER, CF
KURER, V
EPPENBERGER, HM
NIGG, EA
EXPERIENTIA, 1984, 40 (06): : 630 - 630
[6] FLOW CYTOMETRIC CHARACTERIZATION OF CHICKEN B-CELL AND T-CELL ANTIGENS USING MONOCLONAL-ANTIBODIES
EEROLA, E
VEROMAA, T
VAINIO, O
TOIVANEN, P
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1986, 24 (04) : 460 - 460
[7] Nanosensing measurement using novel monoclonal antibodies generated by B-cell targeting
Tomita, M.
Yokota, T.
Wada, M.
Nagaya, M.
Tsumoto, K.
Hirobe, M.
Goda, Y.
FEBS JOURNAL, 2008, 275 : 373 - 373
[8] A rapid screening and production method using a novel mammalian cell display to isolate human monoclonal antibodies
Tomimatsu, Kosuke
Matsumoto, Shin-ei
Tanaka, Hayato
Yamashita, Makiko
Nakanishi, Hidekazu
Teruya, Kiichiro
Kazuno, Saiko
Kinjo, Tomoya
Hamasaki, Takeki
Kusumoto, Ken-ichi
Kabayama, Shigeru
Katakura, Yoshinori
Shirahata, Sanetaka
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 441 (01) : 59 - 64
[9] INVESTIGATION OF NOVEL MONOCLONAL ANTIBODIES DIRECTED AGAINST GLYCOPROTEIN B OF HUMAN CYTOMEGALOVIRUS FOR NEUTRALISATION IN AN IN VITRO INFECTION SYSTEM
Ho, J.
Siddiqui, S.
McIntosh, M.
McLean, G.
Reeves, M.
IRISH JOURNAL OF MEDICAL SCIENCE, 2018, 187 : S352 - S352
[10] Screening and identification of B cell epitope of the nucleocapsid protein in SARS-CoV-2 using the monoclonal antibodies
Yuanyuan Tian
Gaiping Zhang
Hongliang Liu
Peiyang Ding
Rui Jia
Jingming Zhou
Yumei Chen
Yanhua Qi
Jinran Du
Chao Liang
Xifang Zhu
Aiping Wang
Applied Microbiology and Biotechnology, 2022, 106 : 1151 - 1164

← 1 2 3 4 5 →