Intact intracellular tail is critical for proper functioning of the tumor-associated, hypoxia-regulated carbonic anhydrase IX

被引：29

作者：

Hulikova, Alzbeta ^{[1
]}

Zatovicova, Miriam ^{[1
]}

Svastova, Eliska ^{[1
]}

Ditte, Peter ^{[1
]}

Brasseur, Robert ^{[2
]}

Kettmann, Richard ^{[3
]}

Supuran, Claudiu T. ^{[4
]}

Kopacek, Juraj ^{[1
]}

Pastorek, Jaromir ^{[1
]}

Pastorekova, Silvia ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Virol, Bratislava 84505, Slovakia

[2] Fac Sci Agronom Gembloux, Ctr Biophys Mol Numer, B-5030 Gembloux, Belgium

[3] Fac Sci Agronom Gembloux, B-5030 Gembloux, Belgium

[4] Univ Florence, Lab Chim Bioinorgan, I-50019 Florence, Italy

来源：

FEBS LETTERS | 2009年 / 583卷 / 22期

关键词：

Carbonic anhydrase IX; pH regulation; Cell adhesion; Ectodomain shedding; Hypoxia; Acidosis; PROTEIN-PROTEIN INTERACTIONS; DOMAIN; PH; TRANSMEMBRANE; CANCER; INHIBITORS; RESIDUES; ADHESION; BINDING; GROWTHS;

D O I：

10.1016/j.febslet.2009.10.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbonic anhydrase IX (CA IX) is a tumor-associated, hypoxia-induced enzyme involved in pH regulation and cell adhesion. Its catalytically active ectodomain (ECD) is linked to a transmembrane region and a short intracellular (IC) tail. Removal of the IC tail causes intracellular localization of CA IX. Mutations of basic amino acids within IC do not perturb the membrane position, but reduce shedding of the CA IX ectodomain as well as CA IX-mediated cell dissociation. Moreover, they abolish the CA IX capacity to acidify extracellular pH (pHe) and bind CA IX-selective sulfonamide inhibitor in hypoxia. These findings provide the first evidence for the critical contribution of the IC tail to the proper functioning of CA IX. Structured summary: MINT-7293982: E-cadherin (uniprotkb:Q95LE0) and CA IX (genbank_protein_gi:223556027) colocalize (MI:0403) by fluorescence microscopy (MI:0416) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3563 / 3568

页数：6

共 50 条

[21] In Silico Modeling of Carbonic Anhydrase Inhibitor hCA IX-Tumor Associated (Hypoxia)
Shalini Singh
Proceedings of the National Academy of Sciences, India Section A: Physical Sciences, 2012, 82 : 113 - 123
[22] Carbonic anhydrase inhibitors: synthesis and inhibition of cytosolic/tumor-associated carbonic anhydrase isozymes I, II, and IX with bis-sulfamates
Winum, JY
Pastorekova, S
Jakubickova, L
Montero, JL
Scozzafava, A
Pastorek, J
Vullo, D
Innocenti, A
Supuran, CT
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2005, 15 (03) : 579 - 584
[23] Benzimidazole derivatives as potent and isoform selective tumor-associated carbonic anhydrase IX/XII inhibitors
Uslu, Azize Gizem
Maz, Tugce Gur
Nocentini, Alessio
Banoglu, Erden
Supuran, Claudiu T.
Caliskan, Burcu
BIOORGANIC CHEMISTRY, 2020, 95
[24] Carbonic anhydrase inhibitors. Inhibition of tumor-associated isozyme IX by halogenosulfanilamide and halogenophenylaminobenzolamide derivatives
Ilies, MA
Vullo, D
Pastorek, J
Scozzafava, A
Ilies, M
Caproiu, MT
Pastorekova, S
Supuran, CT
JOURNAL OF MEDICINAL CHEMISTRY, 2003, 46 (11) : 2187 - 2196
[25] Coumarin-pyrazoline Hybrids as Selective Inhibitors of the Tumor-associated Carbonic Anhydrase IX and XII
Redij, Aditi
Carradori, Simone
Petreni, Andrea
Supuran, Claudiu T.
Toraskar, Mrunmayee P.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 2023, 23 (10) : 1217 - 1223
[26] Coumarin and Piperazine Conjugates as Selective Inhibitors of the Tumor-associated Carbonic Anhydrase IX and XII Isoforms
Sethi, Aaftaab
Munagalasetty, Sharon
Arifuddin, Mohammed
Carradori, Simone
Supuran, Claudiu T.
Alvala, Ravi
Alvala, Mallika
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 2023, 23 (10) : 1184 - 1191
[27] Carbonic anhydrase IX (CA9) modulates tumor-associated cell migration and invasion
Shin, Hye-Jin
Rho, Seung Bae
Jung, Dae Chul
Han, Inn-Oc
Oh, Eok-Soo
Kim, Joo-Young
JOURNAL OF CELL SCIENCE, 2011, 124 (07) : 1077 - 1087
[28] Carbonic anhydrase inhibitors.: Design of fluorescent sulfonamides as probes of tumor-associated carbonic anhydrase IX that inhibit isozyme IX-mediated acidification of hypoxic tumors
Cecchi, A
Hulikova, A
Pastorek, J
Pastoreková, S
Scozzafava, A
Winum, JY
Montero, JL
Supuran, CT
JOURNAL OF MEDICINAL CHEMISTRY, 2005, 48 (15) : 4834 - 4841
[29] Development of benzene and benzothiazole-sulfonamide analogues as selective inhibitors of the tumor-associated carbonic anhydrase IX
Manzoor, Shoaib
Angeli, Andrea
Zara, Susi
Carradori, Simone
Rahman, Md Ataur
Raza, Md Kausar
Supuran, Claudiu T.
Hoda, Nasimul
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2022, 243
[30] Carbonic anhydrase inhibitors:: Inhibition of the tumor-associated isozymes IX and XII with a library of aromatic and heteroaromatic sulfonamides
Özensoy, Ö
Puccetti, L
Fasolis, G
Arslan, O
Scozzafava, A
Supuran, CT
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2005, 15 (21) : 4862 - 4866

← 1 2 3 4 5 →