Production of Triterpenoid Sapogenins in Hairy Root Cultures of Silene vulgaris

被引：1
|
作者：
Kim, Yeon Bok [1 ,2 ]
Reed, Darwin W. [2 ]
Covello, Patrick S. [2 ]
机构：
[1] Chungnam Natl Univ, Dept Crop Sci, Taejon 305754, South Korea
[2] CNR, Saskatoon, SK S7N 0W9, Canada
来源：
NATURAL PRODUCT COMMUNICATIONS | 2015年 / 10卷 / 11期
关键词：
Agrobacterium rhizogenes; Hairy root culture; Methyl jasmonate; Triterpenoid saponins; Silene vulgaris; AGROBACTERIUM-TUMEFACIENS; ROL GENES; TRANSFORMATION; SAPONINS;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.
引用
收藏
页码：1919 / 1922
页数：4
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