Molecular characterization of Clostridium difficile clinical isolates in a geriatric hospital

被引：5
|
作者：
Poilane, Isabelle
Humeniuk-Ainouz, Christel
Durand, Isabelle
Janoir, Claire
Cruaud, Philippe
Delmee, Michel
Popoff, Michel R.
Collignon, Anne [1 ]
机构：
[1] Hop Jean Verdier Rene Muret, Microbiol Serv, APHP, Bondy, France
[2] Univ Paris 11, Fac Pharm, USC, INRA,EA3534, Chatenay Malabry, France
[3] Catholic Univ Louvain, Microbiol Unit, Brussels, Belgium
[4] Inst Pasteur, Unite Bacteries Anaerobies & Botulisme, Paris, France
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2007年 / 56卷 / 03期
关键词：
D O I：
10.1099/jmm.0.46608-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.
引用
收藏
页码：386 / 390
页数：5
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