Performance and microbial community dynamics in anaerobic co-digestion of chicken manure and corn stover with different modification methods and trace element supplementation strategy

The performance and microbial community dynamics in anaerobic co-digestion (ACoD) of chicken manure and corn stover with different modification methods and trace element supplementation strategy were investigated in this study. KOH and liquid fraction of digestate (LFD) were applied for modification; Fe, Co, Mn, Mo, and Ni were used for supplement. Results showed that the selected trace element was insufficient in the partial or whole digestion process. When trace element supplement was combined with KOH or LFD modifications, the ACoD obtained biomethane yields of 245.3-258.0 and 254.0-261.8 mL(N.gV)S(-1), 26.0%-32.5% and 30.5%-34.5% more than that of the control, respectively. Microbial community analyses indicated that the composition and diversity of archaea and bacteria varied at genus level. Main pathways involved in ACoD were affected accordingly, which in turn affected co-digestion performance. This study demonstrated that the combining modification and trace element supplement could improve the digestion performance and achieve higher biomethane yield.