Fluorescence lifetime correlation spectroscopy

被引：137
|
作者：
Kapusta, Peter
Wahl, Michael
Benda, Ales
Hof, Martin
Enderlein, Jorg
机构：
[1] PicoQuant GmbH, D-12489 Berlin, Germany
[2] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, CR-18223 Prague, Czech Republic
[3] Forschungszentrum Julich Helmholtzgesell, D-52425 Julich, Germany
来源：
JOURNAL OF FLUORESCENCE | 2007年 / 17卷 / 01期
关键词：
FCS; TCSPC; lifetime; correlation; multichannel detection; scattering; afterpulsing;
D O I：
10.1007/s10895-006-0145-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments. The method makes possible to separate the autocorrelation function of individual components of a mixture of fluorophores, as well as purging the result from parasitic contributions like scattered light or detector afterpulsing.
引用
收藏
页码：43 / 48
页数：6
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