Isolation and characterization of 10 polymorphic microsatellite loci from small yellow croaker (Pseudosciaena polyactis)

被引：6
|
作者：
Chen, Song-Lin [1 ]
Xing, Shi-Chao [1 ,2 ]
Xu, Gen-Bo [1 ]
Liao, Xiaolin [1 ]
Yang, Jing-Feng [1 ]
机构：
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
来源：
CONSERVATION GENETICS | 2009年 / 10卷 / 05期
关键词：
Small yellow croaker; Pseudosciaena polyactis; Enriched genomic library; SSR; Microsatellites; EST-LIBRARY;
D O I：
10.1007/s10592-008-9762-3
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis.
引用
收藏
页码：1469 / 1471
页数：3
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