Effect of natural phenols on the catalytic activity of cytochrome P450 2E1

被引:0
|
作者
Mikstacka, R [1 ]
Gnojkowski, J [1 ]
Baer-Dubowska, W [1 ]
机构
[1] Univ Med Sci, Dept Pharmaceut Biochem, PL-60780 Poznan, Poland
关键词
polyphenols; tannic acid; protocatechuic acid; resveratrol; CYP2E1; inhibition kinetics; mechanism-based inhibition;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 0.4 muM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 +/- 3.3 muM showed mixed- and trans-resveratrol competitive inhibition kinetics (K-i = 1 muM and 2.1 muM, respectively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH activity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.
引用
收藏
页码:917 / 925
页数:9
相关论文
共 50 条
  • [41] Cytochrome P450 2E1 responsiveness in the promoter of glutamate-cysteine ligase catalytic subunit
    Nieto, N
    Marí, M
    Cederbaum, AI
    HEPATOLOGY, 2003, 37 (01) : 96 - 106
  • [42] Catalytic activity and quantitation of cytochrome P-450 2E1 in prenatal human brain
    Brzezinski, MR
    Boutelet-Bochan, H
    Person, RE
    Fantel, AG
    Juchau, MR
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1999, 289 (03): : 1648 - 1653
  • [43] BACULOVIRUS EXPRESSION OF HUMAN P450 2E1 AND CYTOCHROME B(5) - SPECTRAL AND CATALYTIC PROPERTIES AND EFFECT OF B(5) ON THE STOICHIOMETRY OF P450 2E1-CATALYZED REACTIONS
    PATTEN, CJ
    KOCH, P
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 317 (02) : 504 - 513
  • [44] Effect of N,N′-dinitrosopiperazine on in vitro expression of human cytochrome P450 2E1
    Zhu, JH
    He, ZM
    Wang, SL
    Chen, ZC
    CHINESE MEDICAL JOURNAL, 2002, 115 (08) : 1229 - 1232
  • [45] Cytochrome P450 2E1 and hyperglycemia-induced liver injury
    Dey, Aparajita
    Kumar, S. Mathan
    CELL BIOLOGY AND TOXICOLOGY, 2011, 27 (04) : 285 - 310
  • [46] Role of Mitochondrial Cytochrome P450 2E1 in Healthy and Diseased Liver
    Massart, Julie
    Begriche, Karima
    Hartman, Jessica H.
    Fromenty, Bernard
    CELLS, 2022, 11 (02)
  • [47] Stereoselective metabolism of enflurane by human liver cytochrome P450 2E1
    Garton, KJ
    Yuen, P
    Meinwald, J
    Thummel, KE
    Kharasch, ED
    DRUG METABOLISM AND DISPOSITION, 1995, 23 (12) : 1426 - 1430
  • [48] Role of cytochrome P450 2E1 in the metabolism of acrylamide and acrylonitrile in mice
    Sumner, SCJ
    Fennell, TR
    Moore, TA
    Chanas, B
    Gonzalez, F
    Ghanayem, BI
    CHEMICAL RESEARCH IN TOXICOLOGY, 1999, 12 (11) : 1110 - 1116
  • [49] Cytochrome p450 2E1 polymorphisms and the risk of gastric cardia cancer
    Cai, Lin
    Zheng, Zong-Li
    Zhang, Zuo-Feng
    WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (12) : 1867 - 1871
  • [50] The Role of Human Cytochrome P450 2E1 in Liver Inflammation and Fibrosis
    Xu, Jun
    Ma, Hsiao-Yen
    Liang, Shuang
    Sun, Mengxi
    Karin, Gabriel
    Koyama, Yukinori
    Hu, Ronglin
    Quehenberger, Oswald
    Davidson, Nicholas O.
    Dennis, Edward A.
    Kisseleva, Tatiana
    Brenner, David A.
    HEPATOLOGY COMMUNICATIONS, 2017, 1 (10) : 1043 - 1057