The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 0.4 muM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 +/- 3.3 muM showed mixed- and trans-resveratrol competitive inhibition kinetics (K-i = 1 muM and 2.1 muM, respectively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH activity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.
机构:Department of Drug Metabolism and Pharmacokinetics, Drug Safety, Sandoz Research Institute, Sandoz Pharmaceuticals Corporation, East Hanover, NJ 07936
PATTEN, CJ
KOCH, P
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机构:Department of Drug Metabolism and Pharmacokinetics, Drug Safety, Sandoz Research Institute, Sandoz Pharmaceuticals Corporation, East Hanover, NJ 07936
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Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R ChinaCent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R China
Zhu, JH
He, ZM
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Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R ChinaCent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R China
He, ZM
Wang, SL
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Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R ChinaCent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R China
Wang, SL
Chen, ZC
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Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R ChinaCent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Peoples R China
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Anna Univ, Div Life Sci, AU KBC Res Ctr, Chennai 600044, Tamil Nadu, IndiaAnna Univ, Div Life Sci, AU KBC Res Ctr, Chennai 600044, Tamil Nadu, India
Dey, Aparajita
Kumar, S. Mathan
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Anna Univ, Div Life Sci, AU KBC Res Ctr, Chennai 600044, Tamil Nadu, IndiaAnna Univ, Div Life Sci, AU KBC Res Ctr, Chennai 600044, Tamil Nadu, India
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Univ Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90095 USA
Fujian Med Univ, Dept Epidemiol, Fuzhou 350004, Fujian Province, Peoples R ChinaUniv Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90095 USA
Cai, Lin
Zheng, Zong-Li
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Fujian Med Univ, Dept Epidemiol, Fuzhou 350004, Fujian Province, Peoples R ChinaUniv Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90095 USA
Zheng, Zong-Li
Zhang, Zuo-Feng
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Univ Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Sch Publ Hlth, Dept Epidemiol, Los Angeles, CA 90095 USA
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Xu, Jun
Ma, Hsiao-Yen
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Ma, Hsiao-Yen
Liang, Shuang
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Liang, Shuang
Sun, Mengxi
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Sun, Mengxi
Karin, Gabriel
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Karin, Gabriel
Koyama, Yukinori
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Koyama, Yukinori
Hu, Ronglin
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Hu, Ronglin
Quehenberger, Oswald
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Quehenberger, Oswald
Davidson, Nicholas O.
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Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Davidson, Nicholas O.
Dennis, Edward A.
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Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA
Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Dennis, Edward A.
Kisseleva, Tatiana
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Univ Calif San Diego, Dept Surg, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA
Kisseleva, Tatiana
Brenner, David A.
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Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, La Jolla, CA 92093 USA